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鼠伤寒沙门氏菌对野生型和免疫缺陷小鼠进行全身感染的转录组和蛋白质组分析

Transcriptome and proteome analysis of Salmonella enterica serovar Typhimurium systemic infection of wild type and immune-deficient mice.

作者信息

Oshota Olusegun, Conway Max, Fookes Maria, Schreiber Fernanda, Chaudhuri Roy R, Yu Lu, Morgan Fiona J E, Clare Simon, Choudhary Jyoti, Thomson Nicholas R, Lio Pietro, Maskell Duncan J, Mastroeni Pietro, Grant Andrew J

机构信息

Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom.

Computer Laboratory, University of Cambridge, JJ Thomson Avenue, Cambridge, United Kingdom.

出版信息

PLoS One. 2017 Aug 10;12(8):e0181365. doi: 10.1371/journal.pone.0181365. eCollection 2017.

DOI:10.1371/journal.pone.0181365
PMID:28796780
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5552096/
Abstract

Salmonella enterica are a threat to public health. Current vaccines are not fully effective. The ability to grow in infected tissues within phagocytes is required for S. enterica virulence in systemic disease. As the infection progresses the bacteria are exposed to a complex host immune response. Consequently, in order to continue growing in the tissues, S. enterica requires the coordinated regulation of fitness genes. Bacterial gene regulation has so far been investigated largely using exposure to artificial environmental conditions or to in vitro cultured cells, and little information is available on how S. enterica adapts in vivo to sustain cell division and survival. We have studied the transcriptome, proteome and metabolic flux of Salmonella, and the transcriptome of the host during infection of wild type C57BL/6 and immune-deficient gp91-/-phox mice. Our analyses advance the understanding of how S. enterica and the host behaves during infection to a more sophisticated level than has previously been reported.

摘要

肠炎沙门氏菌对公众健康构成威胁。目前的疫苗并不完全有效。肠炎沙门氏菌在全身性疾病中致病需要具备在吞噬细胞内的感染组织中生长的能力。随着感染的进展，细菌会面临复杂的宿主免疫反应。因此，为了在组织中持续生长，肠炎沙门氏菌需要对适应性基因进行协调调控。迄今为止，对细菌基因调控的研究主要是利用暴露于人工环境条件或体外培养细胞的方式，而关于肠炎沙门氏菌如何在体内适应以维持细胞分裂和存活的信息却很少。我们研究了野生型C57BL/6和免疫缺陷的gp91-/-phox小鼠感染期间沙门氏菌的转录组、蛋白质组和代谢通量，以及宿主的转录组。我们的分析将对肠炎沙门氏菌与宿主在感染过程中的行为的理解提升到了比以往报道更复杂的水平。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b29/5552096/769c7e05b35f/pone.0181365.g009.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b29/5552096/769c7e05b35f/pone.0181365.g009.jpg

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