Zhang Hui, Li Kun, Wang Yajing, Rehman Mujeeb Ur, Liu Yijiang, Jin Junjie, Peng Junping, Nabi Fazul, Mehmood Khalid, Luo Houqiang, Wang Jiaxiang
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
College of Animal Science, Wenzhou Vocational College of Science and Technology, Wenzhou 325006, People's Republic of China.
J Vet Med Sci. 2017 Oct 7;79(10):1633-1636. doi: 10.1292/jvms.16-0447. Epub 2017 Aug 17.
This study was undertaken to investigate drug resistance in Escherichia coli (E. coli) strains isolated from bamboo rats in Zhejiang province of China. One hundred and fifty-four E. coli strains were isolated from dead bamboo rats. Polymerase chain reaction (PCR) was used to detect the representative genes encoding resistance to commonly used β-lactam antibiotics. Highest resistance was observed for cefradine (24.03%), followed by penicillin (20.78%) and ceftazidime (20.13%). The isolation rates of β-lactam resistance genes were 53.25, 48.70, 15.58 and 14.29% for bla , bla , bla and bla , respectively, while 62 (40.26%) E. coli isolates harbored multiple β-lactam resistance genes. These results also suggested that long term use of these antibiotics leads to antibimicrobial resistance. We believe that this study will provide a guideline for veterinarians and a research basis for examining resistance-encoding genes in other food animals like bamboo rats.
本研究旨在调查从中国浙江省竹鼠分离出的大肠杆菌（E. coli）菌株的耐药性。从死亡的竹鼠中分离出154株大肠杆菌。采用聚合酶链反应（PCR）检测编码对常用β-内酰胺类抗生素耐药性的代表性基因。观察到头孢拉定的耐药率最高（24.03%），其次是青霉素（20.78%）和头孢他啶（20.13%）。bla、bla、bla和bla的β-内酰胺耐药基因分离率分别为53.25%、48.70%、15.58%和14.29%，而62株（40.26%）大肠杆菌分离株携带多个β-内酰胺耐药基因。这些结果还表明，长期使用这些抗生素会导致抗菌药物耐药性。我们相信，本研究将为兽医提供指导，并为检测竹鼠等其他食用动物中的耐药编码基因提供研究基础。
