Yan W, Taylor D E
J Bacteriol. 1987 Jun;169(6):2866-8. doi: 10.1128/jb.169.6.2866-2868.1987.
A circular map of the 208-kilobase IncHII plasmid pHH1508a was constructed by using the restriction endonucleases XbaI, XhoI, and NotI. Tn5 insertion mutagenesis showed that transfer genes were distributed widely over the plasmid map. Analysis of the deletion mutant pDT1178 indicated that genes required for conjugative pilus production are located in a 96.0-kilobase region of the plasmid.
利用限制性内切酶XbaI、XhoI和NotI构建了208千碱基IncHII质粒pHH1508a的环形图谱。Tn5插入诱变表明转移基因广泛分布在质粒图谱上。对缺失突变体pDT1178的分析表明,接合菌毛产生所需的基因位于质粒的一个96.0千碱基区域内。