Xin Min, Cao Mengji, Liu Wenwen, Ren Yingdang, Zhou Xueping, Wang Xifeng
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesBeijing, China.
National Citrus Engineering Research Center, Citrus Research Institute, Southwest UniversityChongqing, China.
Front Microbiol. 2017 Aug 9;8:1514. doi: 10.3389/fmicb.2017.01514. eCollection 2017.
Two novel negative-sense, single-stranded (ss) RNA viruses were identified in watermelon plants and named watermelon crinkle leaf-associated virus 1 and 2 (WCLaV-1 and -2), respectively. The multipartite genomes consist of three RNA molecules of ~6.8, 1.4, and 1.3 kb. The genomes and the deduced proteins of RNA1 and RNA3 show features resembling those of members in the genus and ; however, the predicted proteins encoded by RNA2 are related to the movement protein (MP) in the genus and . Furthermore, these two viruses define a novel clade in the family , order , which is phylogenetically related to the viruses in the above four genera. Moreover, after mechanical inoculation with WCLaV-1 seedlings of the natural host watermelon plants develop crinkling similar to those observed in the field. These findings enhance our understanding of the evolution and the classification of ssRNA viruses.
在西瓜植株中鉴定出两种新型负义单链（ss）RNA病毒，分别命名为西瓜皱叶相关病毒1和2（WCLaV-1和-2）。多分体基因组由三个约6.8、1.4和1.3 kb的RNA分子组成。RNA1和RNA3的基因组及推导的蛋白质显示出与属和属成员相似的特征；然而，RNA2编码的预测蛋白质与属和属中的运动蛋白（MP）相关。此外，这两种病毒在目科中定义了一个新的进化枝，在系统发育上与上述四个属的病毒相关。此外，用WCLaV-1机械接种后，天然寄主西瓜植株的幼苗会出现类似于田间观察到的皱缩现象。这些发现增进了我们对ssRNA病毒进化和分类的理解。
