麻醉大鼠中脑脚桥被盖核氮能系统对心血管的影响
Cardiovascular effects of nitrergic system of the pedunculopontine tegmental nucleus in anesthetized rats.
作者信息
Shafei Mohammad Naser, Nikyar Tahereh, Hosseini Mahmoud, Niazmand Saeed, Paseban Maryam
机构信息
Neurogenic Inflammation Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Iran.
Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
出版信息
Iran J Basic Med Sci. 2017 Jul;20(7):776-782. doi: 10.22038/IJBMS.2017.9009.
OBJECTIVES
Nitric oxide (NO) is an important neurotransmitter in central nervous system involved in central cardiovascular regulation. The presence of NO in the pedunculopontine tegmental (PPT) nucleus has been shown, but its cardiovascular effect has not been determined. In the present study, the cardiovascular effect of NO in the PPT nucleus was evaluated.
MATERIALS AND METHODS
After induction of anesthesia, a polyethylene catheter (PE-50) filled with heparinized saline inserted into the femoral artery, and the blood pressure (BP) and heart rate (HR) were continuously recorded. Animals were then placed in a stereotaxic apparatus and maximum changes of mean arterial pressure (∆MAP) and heart rate (∆HR) after microinjection of two doses of N-nitro-L-arginine methyl ester (L-NAME, 30 and 90 nmol), L-arginine (L-Arg 10 and 50 nmol) and sodium nitroprusside (SNP, 9 and 27 nmol) into the PPT were provided and compared with control group (One-way ANOVA).
RESULTS
Both doses of L-NAME significantly increased ∆MAP compared to control (<0.05 and <0.01, respectively). ∆HR only in higher dose (90 nmol) significantly increased compared to control (<0.05). Two doses of L-Arg (10 and 50 nmol/150 nl) had no significant effect on ∆MAP or ∆HR. Higher dose of SNP (27 nmol) significantly decreased ∆MAP (<0.05) and its both doses significantly decreased ∆HR compared to control (<0.05 and <0.001, respectively). Effect of higher dose on ∆HR was significantly higher than the lower dose (<0.05).
CONCLUSION
Our results show an inhibitory effect of the nitrergic system of the PPT on central cardiovascular system.
目的
一氧化氮（NO）是中枢神经系统中参与中枢心血管调节的重要神经递质。脚桥被盖核（PPT）中已证实存在NO，但其心血管效应尚未确定。在本研究中，评估了NO在PPT核中的心血管效应。
材料与方法
麻醉诱导后，将充满肝素化盐水的聚乙烯导管（PE-50）插入股动脉，连续记录血压（BP）和心率（HR）。然后将动物置于立体定位仪中，向PPT微量注射两剂量的N-硝基-L-精氨酸甲酯（L-NAME，30和90 nmol）、L-精氨酸（L-Arg 10和50 nmol）和硝普钠（SNP，9和27 nmol）后，提供平均动脉压（∆MAP）和心率（∆HR）的最大变化，并与对照组进行比较（单因素方差分析）。
结果
与对照组相比，两剂量的L-NAME均显著增加∆MAP（分别为<0.05和<0.01）。仅高剂量（90 nmol）的∆HR与对照组相比显著增加（<0.05）。两剂量的L-Arg（10和50 nmol/150 nl）对∆MAP或∆HR无显著影响。与对照组相比，高剂量的SNP（27 nmol）显著降低∆MAP（<0.05），其两剂量均显著降低∆HR（分别为<0.05和<0.001）。高剂量对∆HR的影响显著高于低剂量（<0.05）。
结论
我们的结果显示PPT的硝化系统对中枢心血管系统具有抑制作用。
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