Key Laboratory of Meat Processing and Quality Control, Ministry of Education, Synergetic Innovation Center of Food Safety and Nutrition, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Key Laboratory of Meat Processing and Quality Control, Ministry of Education, Synergetic Innovation Center of Food Safety and Nutrition, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Poult Sci. 2017 Sep 1;96(9):3473-3481. doi: 10.3382/ps/pex116.
The selection of broilers for augmented growth rate and breast has brought about wooden-breast (WB) muscle abnormalities, which caused substantial economic losses. The objective of this study was to compare water holding capacity, water mobility and distribution, salt-soluble protein (SSP) content, and protein profiles of normal and WB chicken meat with different additions of NaCl. Thirty WB and 30 normal chicken breasts were selected from a deboning line of a major Chinese processing plant at 2 to 3 h post mortem. Two different meat batters were formulated to 150 mg/g meat protein and different NaCl contents (0%, 1%, 2%, 3%, and 4%). Results indicated that as NaCl contents increased, the cooking loss of meat batters decreased (P < 0.05). Increasing the NaCl content to 3% or more increased the solubility of myofibrillar protein and the extraction of SSPs, which resulted in the improving of cooking yield. Over a range of salt concentrations, normal and WB meat showed different protein profiles, with myosin heavy chain exhibiting a higher intensity at ≥3% salt level. Low-field nuclear magnetic resonance (LF-NMR)revealed an increased T22 and higher P22 in raw WB meat compared to normal meat (P < 0.05). Regarding the meat batters, WB meat batters had reduced T21 and lower immobilized water proportions at low NaCl contents (<2%). After heating, T2 shifted towards higher relaxation times with increasing NaCl contents in meat gels. Meat gels prepared from WB had a lower proportion of water within the myofibrillar protein matrix and a greater proportion of exuded bulk water at NaCl contents <3% (P < 0.05), while at higher NaCl contents the difference was eliminated, thus improving water retention capacity. In conclusion, for raw meat, meat batters and gels, water distribution and mobility of WB exhibited significant differences compared to normal meat. The addition of NaCl affected water mobility and distributions in meat batters, with a level of 3% NaCl eliminating the differences between processed normal and WB meat products.
肉鸡选择增加生长速度和胸部肌肉导致出现木质鸡胸(WB)肌肉异常,这给肉鸡业造成了巨大的经济损失。本研究的目的是比较不同 NaCl 添加量的正常鸡胸肉和 WB 鸡胸肉的持水力、水分流动性和分布、盐溶性蛋白(SSP)含量和蛋白质谱。在屠宰后 2 至 3 小时,从中国主要加工厂的去骨线上选择 30 块 WB 鸡胸肉和 30 块正常鸡胸肉。将两种不同的肉糊制成 150 mg/g 肉蛋白和不同的 NaCl 含量(0%、1%、2%、3%和 4%)。结果表明,随着 NaCl 含量的增加,肉糊的蒸煮损失减少(P<0.05)。将 NaCl 含量增加到 3%或更高会增加肌原纤维蛋白的溶解度和 SSP 的提取,从而提高蒸煮得率。在盐浓度范围内,正常和 WB 肉表现出不同的蛋白质谱,肌球蛋白重链在≥3%盐水平下显示出更高的强度。低场核磁共振(LF-NMR)表明,与正常肉相比,生 WB 肉的 T22 增加,P22 更高(P<0.05)。关于肉糊,WB 肉糊在低盐含量(<2%)下具有更低的 T21 和更低的固定水比例。在加热后,随着肉凝胶中 NaCl 含量的增加,T2 向更高的弛豫时间移动。在 NaCl 含量<3%时,WB 肉凝胶的肌原纤维蛋白基质内的水比例较低,渗出的大块水比例较高(P<0.05),而在较高的 NaCl 含量下,差异消除,从而提高了保水能力。总之,对于生肉、肉糊和凝胶,WB 肉的水分分布和流动性与正常肉有显著差异。NaCl 的添加影响了肉糊中的水分流动性和分布,3%NaCl 的添加消除了加工正常和 WB 肉产品之间的差异。