Abdad Mohammad Y, Abdallah Rita Abou, Karkouri Khalid El, Beye Mamadou, Stenos John, Owen Helen, Unsworth Nathan, Robertson Ian, Blacksell Stuart D, Nguyen Thi-Tien, Nappez Claude, Raoult Didier, Fenwick Stan, Fournier Pierre-Edouard
School of Veterinary and Biomedical Sciences, Murdoch University, South Street, Murdoch 6150, Western Australia, Australia.
Australian Rickettsial Reference Laboratory, Barwon Biomedical Research, Geelong Hospital, Bellerine Street, Geelong 3220, Victoria, Australia.
Int J Syst Evol Microbiol. 2017 Sep;67(9):3156-3161. doi: 10.1099/ijsem.0.001865. Epub 2017 Aug 31.
A rickettsial organism harboured by Amblyomma triguttatum ticks on Barrow Island, Western Australia, was discovered after reports of possible rickettsiosis among local workers. Subsequent isolation of this rickettsia (strain BWI-1) in cell culture and analysis of its phylogenetic, genotypic and phenotypic relationships with type strains of Rickettsia species with standing in nomenclature suggested that it was sufficiently divergent to warrant its classification as a new species. Multiple gene comparison of strain BWI-1 revealed degrees of sequence similarity with Rickettsia raoultii, its closest relative, of 99.58, 98.89, 97.03, 96.93 and 95.73 % for the 16S rRNA, citrate synthase, ompA, ompB and sca4 genes, respectively. Serotyping in mice also demonstrated that strain BWI-1T was distinct from Rickettsia raoultii. Thus, we propose the naming of a new species, Rickettsia gravesii sp. nov., based on its novel genotypic and phenotypic characteristics. Strain BWI-1T was deposited in the ATCC, CSUR and ARRL collections under reference numbers VR-1664, CSUR R172 and RGBWI-1, respectively.
在西澳大利亚州巴罗岛，当地工人中出现可能的立克次体病报告后，人们发现了一种由三盾硬蜱携带的立克次体微生物。随后，该立克次体（菌株BWI-1）在细胞培养中被分离出来，并对其与已命名的立克次体属模式菌株的系统发育、基因型和表型关系进行分析，结果表明它的差异足够大，足以被归类为一个新物种。菌株BWI-1的多基因比较显示，其与最接近的亲属拉乌尔立克次体在16S rRNA、柠檬酸合酶、ompA、ompB和sca4基因上的序列相似程度分别为99.58%、98.89%、97.03%、96.93%和95.73%。在小鼠中的血清分型也表明菌株BWI-1T与拉乌尔立克次体不同。因此，基于其新的基因型和表型特征，我们提议命名一个新物种，即格雷夫斯立克次体（Rickettsia gravesii sp. nov.）。菌株BWI-1T分别以参考编号VR-1664、CSUR R172和RGBWI-1保藏于美国典型培养物保藏中心（ATCC）、法国国家科研中心细菌菌株保藏库（CSUR）和澳大利亚皇家研究实验室（ARRL）。
