Lee Junho, Ha Ji Won
Department of Chemistry, University of Ulsan, 93 Daehak-ro, Nam-gu, Ulsan 44610, Republic of Korea.
Phys Chem Chem Phys. 2017 Sep 20;19(36):24453-24457. doi: 10.1039/c7cp04714b.
Plasmonic gold microrods (AuMRs) are promising optical probes because of direct observation of their in-plane orientations and motions under an optical microscope. However, our understanding of the optical properties of AuMRs is still very limited to the single particle level, and their out-of-plane orientations cannot be resolved by eye with high accuracy. Herein, we present the scattering properties of 1 μm-long AuMRs under a defocused DF microscope. The characteristic defocused scattering patterns of single AuMRs allowed us to resolve both in-plane and out-of-plane angles of single AuMRs. We further demonstrate the feasibility of using the defocused orientation imaging technique to track rotational motions and out-of-plane angles of single AuMRs on synthetic membranes.
等离子体金微棒（AuMRs）是很有前景的光学探针，因为可以在光学显微镜下直接观察其面内取向和运动。然而，我们对AuMRs光学性质的理解在单粒子水平上仍然非常有限，并且它们的面外取向无法通过肉眼高精度分辨。在此，我们展示了1μm长的AuMRs在离焦暗场（DF）显微镜下的散射特性。单个AuMRs的特征离焦散射图案使我们能够分辨单个AuMRs的面内和面外角度。我们进一步证明了使用离焦取向成像技术跟踪合成膜上单个AuMRs的旋转运动和面外角度的可行性。
