钴胺素辅酶在光合绿藻普通小球藻中的存在情况。

Occurrence of Cobalamin Coenzymes in the Photosynthetic Green Alga, Chlorella vulgaris.

作者信息

Watanabe Fumio, Abe Katsuo, Takenaka Shigeo, Tamura Yoshiyuki, Maruyama Isao, Nakano Yoshihisa

机构信息

a Department of Food and Nutrition , Kochi Women's University , Kochi 780 , Japan.

b Department of Nutrition and Food Science , Hagoromo-gakuen College , Sakai, Osaka 592 , Japan.

出版信息

Biosci Biotechnol Biochem. 1997 Jan;61(5):896-897. doi: 10.1271/bbb.61.896.

DOI:10.1271/bbb.61.896

PMID:28862557

Abstract

To analyze cobalamin metabolism in photosynthetic green algae, the effects of cobalamin on growth of Chlorella vulgaris C-30 were studied and the algal cobalamin contents were assayed. Cobalamin significantly stimulated growth of the Chlorella cells, but biologically inactive cobalamin analogues did not. Chlorella grown in a cobalamin-free medium (control) contained cobalamin coenzymes, 5'-deoxyadenosylcobalamin (7.95 ± 0.31 ng/g wet weight) and methylcobalamin (2.72 ± 0.45 ng/g wet weight), of which the levels were increased significantly in cobalamin-supplemented cells. These results indicate that the alga has ability to take up exogenous cobalamin and synthesize the coenzyme forms.

摘要

为了分析光合绿藻中的钴胺素代谢，研究了钴胺素对普通小球藻C-30生长的影响，并测定了藻类中的钴胺素含量。钴胺素显著刺激了小球藻细胞的生长，但无生物活性的钴胺素类似物则没有这种作用。在不含钴胺素的培养基（对照）中生长的小球藻含有钴胺素辅酶，即5'-脱氧腺苷钴胺素（7.95±0.31 ng/g湿重）和甲基钴胺素（2.72±0.45 ng/g湿重），在添加钴胺素的细胞中，这些辅酶的水平显著增加。这些结果表明，该藻类具有摄取外源钴胺素并合成辅酶形式的能力。