Murata Yusuke, Oka Ayana, Iseki Ayaka, Mori Masayoshi, Ohe Kenji, Mine Kazunori, Enjoji Munechika
Department of Pharmacotherapeutics, Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1, Nanakuma, Jonan-ku, Fukuoka, 814-0180, Japan.
Department of Pharmacotherapeutics, Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1, Nanakuma, Jonan-ku, Fukuoka, 814-0180, Japan.
Neurosci Res. 2018 Jun;131:45-51. doi: 10.1016/j.neures.2017.08.008. Epub 2017 Sep 1.
Previous studies have indicated that sleep deprivation negatively affects hippocampal neurogenesis, which may explain the reason for the relation between sleep loss and depression. Increasing evidence indicates that the hippocampus is anatomically and functionally segregated along a dorsolateral (cognitive function)/ventromedial (control for mood and stress response) axis. Thus, the present study was conducted to elucidate regional differences in the adverse effects of sleep deprivation on hippocampal neurogenesis. Male Sprague-Dawley rats were subjected to sleep deprivation using the "platform on the water" method for 24- or 72-h. Quantification of hippocampal cell proliferation and immature newborn neurons was stereologically estimated using immunostaining with Ki-67 and doublecortin (DCX), respectively, by optical fractionator method. A consecutive three days of sleep deprivation significantly reduced the density of Ki-67- and DCX-immunopositive cells both in the dorsal and ventral hippocampal subgranular zone and the decrease in DCX-labeled cells was more pronounced in the ventral hippocampus than in dorsal region. Our results indicate that prolonged sleep deprivation decreases hippocampal cell proliferation and neurogenesis in both the dorsal and ventral dentate gyrus. Future studies will be needed to clarify the impact of sleep deprivation-induced decreases in hippocampal neurogenesis on the development of depression.
先前的研究表明，睡眠剥夺会对海马体神经发生产生负面影响，这可能解释了睡眠不足与抑郁症之间存在关联的原因。越来越多的证据表明，海马体在解剖学和功能上沿背外侧（认知功能）/腹内侧（情绪和应激反应控制）轴呈分离状态。因此，本研究旨在阐明睡眠剥夺对海马体神经发生的不利影响的区域差异。采用“水上平台”法对雄性斯普拉格-道利大鼠进行24小时或72小时的睡眠剥夺。分别使用Ki-67免疫染色和双皮质素（DCX）免疫染色，通过光学分割法对海马体细胞增殖和未成熟新生神经元进行立体定量分析。连续三天的睡眠剥夺显著降低了背侧和腹侧海马颗粒下区Ki-67免疫阳性细胞和DCX免疫阳性细胞的密度，且腹侧海马中DCX标记细胞的减少比背侧区域更明显。我们的结果表明，长期睡眠剥夺会降低背侧和腹侧齿状回的海马体细胞增殖和神经发生。未来需要进一步研究来阐明睡眠剥夺引起的海马体神经发生减少对抑郁症发展的影响。
