基于原卟啉IX的光动力疗法在促进结肠癌细胞损伤和凋亡中的作用及其机制
[Role of PpIX-based photodynamic therapy in promoting the damage and apoptosis of colorectal cancer cell and its mechanisms].
作者信息
Ouyang Guoqing, Liu Zhipeng, Xiong Li, Chen Xiang, Li Qinglong, Huang He, Lin Liangwu, Miao Xiongying, Ma Lun, Chen Wei, Wen Yu
机构信息
Department of General Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Department of Histology and Embryology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
出版信息
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2017 Aug 28;42(8):874-881. doi: 10.11817/j.issn.1672-7347.2017.08.002.
To explore the effects of protoporphyrin IX (PpIX)-mediated photodynamic therapy (PDT) on induction of apoptosis and death in colon cancer cell and the underlying mechanisms. Methods: The cell killing effect of PDT on HCT116 cell was determined by cell counting kit (CCK). The cells were divided into a control group, a single light group, a single PpIX group, and a PDT group. Hoechst 33342 and flow cytometry was used to assess the cell apoptosis. Western blot was employed to analyze the expressions of bcl-2, bax, and caspase-3. Reactive oxygen species (ROS) was detected by flow cytometry. Results: The viability of HCT116 cell was decreased gradually with the increase of irradiation dose (P<0.05). Compared to the other 3 groups, ROS production, the number of apoptotic cells and the protein expressions of bax and caspase-3 in PDT group increased, while bcl-2 expression was decreased (P<0.05). Conclusion: PpIX-mediated PDT can enhance the apoptosis in HCT116 cell, which may be related to mitochondrial apoptosis pathway.
探讨原卟啉Ⅸ（PpIX）介导的光动力疗法（PDT）对结肠癌细胞凋亡和死亡的诱导作用及其潜在机制。方法：采用细胞计数试剂盒（CCK）检测PDT对HCT116细胞的杀伤作用。将细胞分为对照组、单纯光照组、单纯PpIX组和PDT组。采用Hoechst 33342和流式细胞术评估细胞凋亡。采用蛋白质免疫印迹法分析bcl-2、bax和caspase-3的表达。通过流式细胞术检测活性氧（ROS）。结果：随着照射剂量的增加，HCT116细胞活力逐渐降低（P<0.05）。与其他3组相比，PDT组ROS生成量、凋亡细胞数量以及bax和caspase-3的蛋白表达增加，而bcl-2表达降低（P<0.05）。结论：PpIX介导的PDT可增强HCT116细胞凋亡，这可能与线粒体凋亡途径有关。
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