Wanlop Atcharaphan, Wongsawad Chalobol, Prattapong Pongphol, Wongsawad Pheravut, Chontananarth Thapana, Chai Jong-Yil
Applied Parasitology Research Laboratory, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Environmental Science Research Center (ESRC), Chiang Mai University, Chiang Mai 50200, Thailand.
Korean J Parasitol. 2017 Aug;55(4):445-449. doi: 10.3347/kjp.2017.55.4.445. Epub 2017 Aug 31.
The prevalence of Centrocestus formosanus metacercariae was investigated in ornamental fish purchased from a pet shop in Chiang Mai, Thailand, including Carassius auratus (goldfish), Cyprinus carpio (Koi), Poecilia latipinna (Sailfin Molly), Danio rerio (Zebrafish), and Puntigrus tetrazona (Tiger barb). The parasite species was identified by the morphology of worms as well as by a molecular approach using ITS2. The results showed that 50 (33.3%) of 150 fish examined were infected with the metacercariae. The highest prevalence was found in C. auratus (83.3%), and the highest intensity was noted in C. carpio (70.8 metacercariae/fish). The most important morphological character was the presence of 32-34 circumoral spines on the oral sucker. The phylogenetic studies using the rRNA ITS2 region revealed that all the specimens of C. formosanus in this study were grouped together with C. formosanus in GenBank database. This is the first report on ornamental fish, C. carpio, P. latipinna, D. rerio, and P. tetrazona, taking the role of second intermediate hosts of C. formosanus in Thailand. Prevention and control of metacercarial infection in ornamental fish is urgently needed.
在泰国清迈一家宠物店购买的观赏鱼中，调查了台湾棘带吸虫后尾蚴的感染率，这些观赏鱼包括鲫（金鱼）、鲤（锦鲤）、帆鳍玛丽鱼、斑马鱼和虎皮鱼。通过虫体形态以及使用ITS2的分子方法对寄生虫种类进行了鉴定。结果显示，在检查的150条鱼中，有50条（33.3%）感染了后尾蚴。感染率最高的是鲫（83.3%），感染强度最高的是鲤（70.8个后尾蚴/鱼）。最重要的形态特征是口吸盘上有32 - 34个口周棘。利用rRNA ITS2区域进行的系统发育研究表明，本研究中所有台湾棘带吸虫标本与GenBank数据库中的台湾棘带吸虫归为一类。这是关于观赏鱼鲤、帆鳍玛丽鱼、斑马鱼和虎皮鱼在泰国作为台湾棘带吸虫第二中间宿主的首次报道。迫切需要预防和控制观赏鱼的后尾蚴感染。
