Lu Kun, Hu Jinyuan, Gao Shixiang, Mao Liang
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, China.
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, China.
Environ Pollut. 2017 Dec;231(Pt 1):863-870. doi: 10.1016/j.envpol.2017.08.093. Epub 2017 Sep 25.
In this study, we systematically investigated the effect of multiwall carbon nanotubes (MWCNTs) on the removal of tetrabromobisphenol A (TBBPA) mediated by horseradish peroxidase (HRP) at varying important conditions. The results suggested that the presence of MWCNTs significantly enhanced the removal of TBBPA mediated by HRP and the reaction rate constant was linear with the MWCNTs dosage. The enhancement of MWCNTs on the HRP-mediated reaction was attributed to two facts, one is that MWCNTs protected HRP from inactivation, the other is that the presence of MWCNTs made the homogeneous reaction of TBBPA be heterogeneous reaction by adsorbing TBBPA on its surface. Moreover, the influence of MWCNTs on TBBPA products distribution was further elucidated. We found that the species of reaction product had no difference between the HRP-mediated systems with and without the presence of MWCNTs. However, the presence of MWCNTs significantly decreased the yields of each product. These results give insight into the role of MWCNTs in HRP-mediated TBBPA reactions and provide theoretical foundation for potential development of novel enzymatic methods to control TBBPA contamination. MWCNTs enhanced the removal of TBBPA mediated by HRP/HO, because it protected HRP from inactivation and adsorbed TBBPA on its surface to form a heterogeneous reaction process.
在本研究中，我们系统地研究了多壁碳纳米管（MWCNTs）在不同重要条件下对辣根过氧化物酶（HRP）介导的四溴双酚A（TBBPA）去除效果的影响。结果表明，MWCNTs的存在显著增强了HRP介导的TBBPA去除效果，且反应速率常数与MWCNTs用量呈线性关系。MWCNTs对HRP介导反应的增强作用归因于两个方面，一是MWCNTs保护HRP不被失活，二是MWCNTs的存在通过将TBBPA吸附在其表面使TBBPA的均相反应变为非均相反应。此外，进一步阐明了MWCNTs对TBBPA产物分布的影响。我们发现，在有和没有MWCNTs存在的HRP介导体系中，反应产物的种类没有差异。然而，MWCNTs的存在显著降低了每种产物的产率。这些结果深入了解了MWCNTs在HRP介导的TBBPA反应中的作用，并为开发控制TBBPA污染的新型酶法提供了理论基础。MWCNTs增强了HRP/HO介导的TBBPA去除效果，因为它保护HRP不被失活，并将TBBPA吸附在其表面形成非均相反应过程。
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