State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, 288 Nanjing Road, Tianjin 300020, PR China.
State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, 288 Nanjing Road, Tianjin 300020, PR China.
Cytokine. 2017 Nov;99:124-131. doi: 10.1016/j.cyto.2017.08.024. Epub 2017 Sep 5.
Primary immune thrombocytopenia (ITP) is a disease of autoimmunity in which there are Th1/Th2 imbalance and disordered cytokine profiles. CXC chemokine ligand 16 (CXCL16) was proved to implicate in some autoimmune diseases. Our research aimed to determine plasma soluble CXCL16 (sCXCL16) levels and its effects in ITP. We used ELISA to measure plasma sCXCL16, IFN-γ and IL-4 and flow cytometry to determine expression of CXCR6 on lymphocyte subsets. We used real-time PCR to detect the CXCL16 and CXCR6 mRNA expression. Additionally, plasma sCXCL16, CXCL16 and CXCR6 mRNA levels of 8 patients were monitored before and after treatment. We found that patients with active ITP had higher circulating sCXCL16 in plasma than healthy controls and patients in remission. Meanwhile, negative relationships between sCXCL16 and platelet count, IL-4 and positive relationships between sCXCL16 and IFN-γ, IFN-γ/IL-4 ratio were observed. Besides, expression of CXCR6 on lymphocyte subsets and mRNA levels of CXCL16 and CXCR6 were all increased in active ITP. Additionally, plasma sCXCL16 and IFN-γ levels and CXCR6 mRNA expression were down-regulated after effective treatment compared with those before treatment. Thus, increased plasma sCXCL16 might be implicated in the pathogenesis of ITP and have a relationship with Th1/Th2 imbalance.
原发性免疫性血小板减少症（ITP）是一种自身免疫性疾病，存在 Th1/Th2 失衡和细胞因子谱紊乱。趋化因子（C-X-C 基序）配体 16（CXCL16）已被证明与一些自身免疫性疾病有关。我们的研究旨在确定血浆可溶性 CXCL16（sCXCL16）水平及其在 ITP 中的作用。我们使用 ELISA 法测量血浆 sCXCL16、IFN-γ 和 IL-4，并用流式细胞术测定淋巴细胞亚群上 CXCR6 的表达。我们使用实时 PCR 检测 CXCL16 和 CXCR6 mRNA 的表达。此外，还监测了 8 例患者治疗前后的血浆 sCXCL16、CXCL16 和 CXCR6 mRNA 水平。我们发现，活动性 ITP 患者的循环 sCXCL16 高于健康对照组和缓解期患者。同时，sCXCL16 与血小板计数呈负相关，与 IL-4 呈负相关，与 IFN-γ 呈正相关，与 IFN-γ/IL-4 比值呈正相关。此外，淋巴细胞亚群上 CXCR6 的表达和 CXCL16 和 CXCR6 的 mRNA 水平在活动性 ITP 中均升高。此外，与治疗前相比，有效治疗后血浆 sCXCL16 和 IFN-γ 水平及 CXCR6 mRNA 表达均下调。因此，增加的血浆 sCXCL16 可能与 ITP 的发病机制有关，并与 Th1/Th2 失衡有关。
