Sobeh Mansour, Mahmoud Mona F, Abdelfattah Mohamed A O, El-Beshbishy Hesham A, El-Shazly Assem M, Wink Michael
Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, 69120-Heidelberg, Germany.
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt.
Phytomedicine. 2017 Sep 15;33:36-42. doi: 10.1016/j.phymed.2017.07.003. Epub 2017 Jul 3.
Liver diseases and diabetes are serious health disorders associated with oxidative stress and ageing. Some plant polyphenols can lower the risk of these diseases.
We investigated the phytochemical profiling of a root extract from Ximenia americana var. caffra using HPLC-PDA-ESI-MS/MS. The antioxidant activities in vitro were investigated. The hepatoprotective activities were studied in rat models with d-galactosamine (d-GaIN)-induced hepatotoxicity and the antidiabetic activities in STZ-diabetic rats were also investigated.
HPLC-PDA-ESI-MS/MS was used to identify plant phenolics. The antioxidant activities in vitro were determined using DPPH and FRAP assays. The in vivo hepatoprotective activities were determined for d-GaIN-induced hepatotoxicity in rats. We determined the liver markers alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma-glutamyltransferase (GGT), liver peroxidation product malondialdehyde (MDA), glutathione content (GSH), albumin and total bilirubin concentration. The histopathological changes in rat liver were also studied. The antidiabetic activities were also investigated in STZ-diabetic rats and serum glucose, serum insulin hormone, and lipid peroxides were determined.
The root extract is rich in tannins with 20 compounds including a series of stereoisomers of (epi)catechin, (epi)catechin-(epi)catechin, (epi)catechin-(epi)catechin-(epi)catechin, and their galloyl esters. Promising antioxidant potential was observed in vitro in DPPH assay with EC of 6.5 µg extract / 26 µg raw material and in FRAP assay with 19.54 mM FeSO compared with ascorbic acid (EC of 2.92 µg/ml) and quercetin (FeSO 24.04 mM/mg), respectively. Significant reduction of serologic enzymatic markers and hepatic oxidative stress markers such as ALT, AST, MDA, GGT, and total bilirubin, as well as elevation of GSH and albumin were observed in rats with d-galactosamine-induced liver damage treated with the extract. These findings agree with a histopathological examination suggesting a hepatoprotective potential for the root extract. The root extract can mediate an antidiabetic effect by reducing elevated blood glucose and serum lipid peroxides levels and by increasing insulin in STZ-diabetic rats by -107, -31.1, +11.3%, respectively.
The results of this study suggest that the tannin-rich extract from Ximenia americana var. caffra could be an interesting candidate for the treatment of several health disorders associated with oxidative stress such as hepatocellular injury and diabetes.
肝脏疾病和糖尿病是与氧化应激及衰老相关的严重健康问题。一些植物多酚可降低这些疾病的风险。
我们使用高效液相色谱-光电二极管阵列-电喷雾串联质谱（HPLC-PDA-ESI-MS/MS）研究了美国西门木（Ximenia americana var. caffra）根提取物的植物化学特征。研究了其体外抗氧化活性。在d-半乳糖胺（d-GaIN）诱导的肝毒性大鼠模型中研究了其肝脏保护活性，并在链脲佐菌素诱导的糖尿病大鼠中研究了其抗糖尿病活性。
使用HPLC-PDA-ESI-MS/MS鉴定植物酚类物质。使用DPPH和FRAP试验测定体外抗氧化活性。测定d-GaIN诱导的大鼠肝毒性的体内肝脏保护活性。我们测定了肝脏标志物丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）和γ-谷氨酰转移酶（GGT）、肝脏过氧化产物丙二醛（MDA）、谷胱甘肽含量（GSH）、白蛋白和总胆红素浓度。还研究了大鼠肝脏的组织病理学变化。在链脲佐菌素诱导的糖尿病大鼠中也研究了抗糖尿病活性，并测定了血糖、血清胰岛素激素和脂质过氧化物。
根提取物富含单宁，含有20种化合物，包括一系列（表）儿茶素、（表）儿茶素-（表）儿茶素、（表）儿茶素-（表）儿茶素-（表）儿茶素的立体异构体及其没食子酰酯。在DPPH试验中，提取物的半数有效浓度（EC）为6.5μg提取物/26μg原料，在FRAP试验中为19.54mM硫酸亚铁，与抗坏血酸（EC为2.92μg/ml）和槲皮素（硫酸亚铁24.04mM/mg）相比，体外观察到有良好的抗氧化潜力。在用提取物治疗的d-半乳糖胺诱导的肝损伤大鼠中，观察到血清酶标志物和肝脏氧化应激标志物如ALT、AST、MDA、GGT和总胆红素显著降低，以及GSH和白蛋白升高。这些发现与组织病理学检查结果一致，表明根提取物具有肝脏保护潜力。根提取物可通过降低链脲佐菌素诱导的糖尿病大鼠的血糖和血清脂质过氧化物水平，并使胰岛素增加11.3%，分别降低107和31.1，从而介导抗糖尿病作用。
本研究结果表明，美国西门木变种根的富含单宁的提取物可能是治疗与氧化应激相关的几种健康问题如肝细胞损伤和糖尿病的一个有吸引力候选物。
Zotero 插件