Iancu T C, Ward R J, Peters T J
Division of Clinical Cell Biology, MRC Clinical Research Centre, Harrow, Middlesex, England.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1987;53(4):208-17. doi: 10.1007/BF02890245.
Rats fed a carbonyl iron-supplemented diet for 4-15 months were studied for iron content and morphologic changes in the liver, spleen, intestinal mucosa, pancreas and heart. All organs had an increased iron content measured by atomic absorption, with the highest concentrations in the liver and spleen. The periportal distribution of stored iron in the liver was similar to that in human hemochromatosis. In animals treated beyond 6 months Kupffer cells and sinusoidal lining cells also showed cytosiderosis. Electron microscopy provided information on ferritin and hemosiderin content and distribution within parenchymal and sinusoidal cells of the liver but no excessive fibrosis was found. Except for the spleen, the other organs showed less iron deposition. Iron-filled lysosomes (siderosomes) were found in macrophages in the intestinal lamina propria and pancreas, as well as in enterocytes, pancreatic acinar cells and heart muscle cells. Heavily iron-laden siderosomes had increased membrane instability which was demonstrated both morphologically and by measurements of latent lysosomal enzyme activities. Even though cirrhosis was not found, the distribution pattern of accumulated storage iron and lysosomal lability indicated that the carbonyl iron-fed rat is a suitable experimental model for human hemochromatosis.
对喂食补充羰基铁饮食4至15个月的大鼠的肝脏、脾脏、肠黏膜、胰腺和心脏的铁含量及形态学变化进行了研究。通过原子吸收法测定，所有器官的铁含量均增加，肝脏和脾脏中的浓度最高。肝脏中储存铁的门周分布与人类血色素沉着症相似。在治疗超过6个月的动物中，库普弗细胞和窦状隙衬里细胞也出现了细胞含铁血黄素沉着。电子显微镜提供了关于肝脏实质细胞和窦状隙细胞内铁蛋白和含铁血黄素含量及分布的信息，但未发现过度纤维化。除脾脏外，其他器官的铁沉积较少。在肠固有层和胰腺的巨噬细胞以及肠上皮细胞、胰腺腺泡细胞和心肌细胞中发现了充满铁的溶酶体（含铁小体）。铁负荷重的含铁小体膜稳定性增加，这在形态学上以及通过潜在溶酶体酶活性的测量都得到了证实。尽管未发现肝硬化，但蓄积的储存铁的分布模式和溶酶体的不稳定性表明，喂食羰基铁的大鼠是人类血色素沉着症的合适实验模型。
