Liu Fang, Pei Fei, Mariga Alfred Mugambi, Gao Le, Chen Guitang, Zhao Liyan
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, China.
Department of Dairy and Food Science and Technology, Egerton University, Egerton, Kenya.
J Food Drug Anal. 2015 Dec;23(4):630-635. doi: 10.1016/j.jfda.2014.06.008. Epub 2015 Jan 2.
Orthogonal experiment was applied to optimize the water extraction parameters of zinc from Flammulina velutipes, and then the extracts were separated by membrane filter (0.45 μm) and D101 macroporous resin. Six different species of Zn were obtained and the Zn content of various species were determined by flame atomic absorption spectrometry. The optimized conditions for the extraction of Zn were: ratio of dried material to water, 1:30; extraction temperature, 75°C; extraction time, 120 minutes. About 34.43 μg Zn was extracted from 1 g dried F. velutipes powder under the optimal conditions. The recovery value for Zn was 96.5% with a low relative standard deviation. In addition, the content of the organic state of Zn was more than that of the inorganic state, and most of the organic state Zn was found in the polysaccharide and protein fractions.
采用正交试验优化金针菇中锌的水提取参数，然后将提取物通过膜过滤器（0.45μm）和D101大孔树脂进行分离。得到了六种不同形态的锌，并通过火焰原子吸收光谱法测定了各种形态锌的含量。锌提取的优化条件为：料水比1:30；提取温度75℃；提取时间120分钟。在最佳条件下，从1g金针菇干粉末中可提取约34.43μg锌。锌的回收率为96.5%，相对标准偏差较低。此外，锌的有机态含量高于无机态，且大部分有机态锌存在于多糖和蛋白质组分中。
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