通过数字图像分析同时测定商业产品中的一些常见食用色素。
Simultaneous determination of some common food dyes in commercial products by digital image analysis.
作者信息
Sorouraddin Mohammad-Hossein, Saadati Masoud, Mirabi Fariba
机构信息
Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran; Department of Science, Ardabil Allameh Tabatabaei Campus, Farhangian University, Iran.
出版信息
J Food Drug Anal. 2015 Sep;23(3):447-452. doi: 10.1016/j.jfda.2014.10.007. Epub 2015 Jan 3.
A simple and relatively fast image-analysis method using digital images, obtained with a flatbed scanner, has been described. The method was used for the simultaneous determination of four common food dyes, namely, carmoisine, brilliant blue, sunset yellow, and quinoline yellow, in binary mixtures in commercial products without a need for any prior separation steps. The results obtained were validated against a standard high-performance liquid chromatography method and a good agreement was obtained. The parameters affecting the experimental results were optimized. Under the optimal conditions, the method provided acceptable linear ranges (20-250 mg/L) with correlation coefficients higher than 0.998, suitable precision (relative standard deviation ≤ 4.5%), and limits of detection between 4.82 and 8.05 mg/L.
本文描述了一种使用平板扫描仪获取数字图像的简单且相对快速的图像分析方法。该方法用于同时测定市售产品二元混合物中的四种常见食用色素，即胭脂红、亮蓝、日落黄和喹啉黄，无需任何预先分离步骤。将所得结果与标准高效液相色谱法进行验证，结果吻合良好。对影响实验结果的参数进行了优化。在最佳条件下，该方法提供了可接受的线性范围（20 - 250 mg/L），相关系数高于0.998，具有合适的精密度（相对标准偏差≤4.5%），检测限在4.82至8.05 mg/L之间。
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