Liu Lijun, Song Yuan, Xu Jun, Li Dongming, Li Gaopeng, An Lizhe
Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730000, China.
Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730000, China.
Gene. 2017 Dec 15;636:1-16. doi: 10.1016/j.gene.2017.09.015. Epub 2017 Sep 11.
Epigenetic modifications regulate plant genes to cope with a variety of environmental stresses. Chorispora bungeana is an alpine subnival plant with strong tolerance to multiple abiotic stresses, especially cold stress. In this study, we characterized the alcohol dehydrogenase 1 gene from Chorispora bungeana, CbADH1, that is up-regulated in cold conditions. Overexpression of CbADH1 in Arabidopsis thaliana improved cold tolerance, as indicated by a decreased lethal temperature (LT50). Chromatin immunoprecipitation assays showed that histone H3 is removed from the promoter region and the middle-coding region of the gene. H3K9 acetylation and H3K4 trimethylation increased throughout the gene and in the proximal promoter region, respectively. Moreover, increased Ser5P and Ser2P polymerase II accumulation further indicated changes in the transcription initiation and elongation of CbADH1 were due to the cold stress. Taken together, our results suggested that CbADH1 is highly expressed during cold stress, and is regulated by epigenetic modifications. This study expands our understanding of the regulation of gene expression by epigenetic modifications in response to environmental cues.
表观遗传修饰调控植物基因以应对各种环境胁迫。 Bungeana Chorispora是一种高山亚冰雪植物，对多种非生物胁迫具有较强的耐受性，尤其是冷胁迫。在本研究中，我们鉴定了来自Bungeana Chorispora的乙醇脱氢酶1基因CbADH1，该基因在寒冷条件下上调。在拟南芥中过表达CbADH1提高了耐寒性，致死温度（LT50）降低表明了这一点。染色质免疫沉淀分析表明，组蛋白H3从该基因的启动子区域和中间编码区域被去除。H3K9乙酰化和H3K4三甲基化分别在整个基因和近端启动子区域增加。此外，Ser5P和Ser2P聚合酶II积累的增加进一步表明，CbADH1转录起始和延伸的变化是由冷胁迫引起的。综上所述，我们的结果表明CbADH1在冷胁迫期间高度表达，并受表观遗传修饰调控。本研究扩展了我们对表观遗传修饰响应环境信号调控基因表达的理解。
