Forrest B D
University of Adelaide, Department of Medicine, Royal Adelaide Hospital, South Australia.
Lancet. 1988 Jan 16;1(8577):81-3. doi: 10.1016/s0140-6736(88)90284-x.
The intestinal immune response of volunteers given the oral vaccine Salmonella typhi Ty21a was assessed with a new immunoassay which measures specific antibody secretion by peripheral blood lymphocytes (PBL). Peak IgA, IgG, and IgM production by PBL occurred on day 7 after the start of vaccination. Peak antibody secretion by PBL occurred several days earlier than the serum antibody peak. All volunteers showed a specific PBL antibody response, but serum or intestinal immune responses, when demonstrable, were highly variable. Peak PBL IgA antibody response correlated with degree of rise in IgA antibody in serum (p = 0.00098) and the intestinal fluid (p = 0.0024). The assay is a useful means of measuring humoral immune response at a mucosal surface after local administration of antigen.
采用一种新的免疫测定法评估了口服伤寒疫苗Ty21a的志愿者的肠道免疫反应,该方法可测量外周血淋巴细胞(PBL)分泌的特异性抗体。接种疫苗开始后第7天,PBL产生的IgA、IgG和IgM达到峰值。PBL分泌抗体的峰值比血清抗体峰值提前几天出现。所有志愿者均表现出特异性PBL抗体反应,但血清或肠道免疫反应(若可检测到)变化很大。PBL IgA抗体反应峰值与血清(p = 0.00098)和肠液中(p = 0.0024)IgA抗体的升高程度相关。该测定法是在局部给予抗原后测量黏膜表面体液免疫反应的一种有用方法。