1 EA 2496, Laboratory Orofacial Pathologies, Imaging and Biotherapies, School of Dentistry, Sorbonne Paris Cité, University Paris Descartes , Paris, France .
2 Department of Pathology and Oral Medicine, Dental Faculty, University of Chile , Santiago, Chile .
Stem Cells Dev. 2017 Dec 1;26(23):1682-1694. doi: 10.1089/scd.2017.0115. Epub 2017 Nov 3.
Neural crest (NC) cells are a migratory, multipotent population giving rise to numerous lineages in the embryo. Their plasticity renders attractive their use in tissue engineering-based therapies, but further knowledge on their in vivo behavior is required before clinical transfer may be envisioned. We here describe the isolation and characterization of a new mouse embryonic stem (ES) line derived from Wnt1-CRE-R26 Rosa blastocyst and show that it displays the characteristics of typical ES cells. Further, these cells can be efficiently directed toward an NC stem cell-like phenotype as attested by concomitant expression of NC marker genes and Tomato fluorescence. As native NC progenitors, they are capable of differentiating toward typical derivative phenotypes and interacting with embryonic tissues to participate in the formation of neo-structures. Their specific fluorescence allows purification and tracking in vivo. This cellular tool should facilitate a better understanding of the mechanisms driving NC fate specification and help identify the key interactions developed within a tissue after in vivo implantation. Altogether, this novel model may provide important knowledge to optimize NC stem cell graft conditions, which are required for efficient tissue repair.
神经嵴（NC）细胞是一种迁移的、多能性的群体，在胚胎中产生许多谱系。它们的可塑性使得它们在基于组织工程的治疗中具有吸引力，但在临床转移之前，需要进一步了解它们的体内行为。我们在这里描述了一种从 Wnt1-CRE-R26 Rosa 囊胚中分离和表征的新的小鼠胚胎干细胞（ES）系，并表明它显示出典型的 ES 细胞特征。此外，这些细胞可以有效地被诱导为具有 NC 干细胞样表型，这表现在同时表达 NC 标记基因和番茄荧光。作为天然的 NC 祖细胞，它们能够向典型的衍生表型分化，并与胚胎组织相互作用，参与新结构的形成。它们的特异性荧光允许在体内进行纯化和跟踪。这种细胞工具应该有助于更好地理解驱动 NC 命运特化的机制，并有助于识别体内植入后组织内发展的关键相互作用。总之，这种新型模型可能为优化 NC 干细胞移植条件提供重要的知识，这是有效组织修复所必需的。
