间充质干细胞移植调节心肌梗死大鼠中Cx43和Cx45的表达。
Transplantation of mesenchymal stem cells modulated Cx43 and Cx45 expression in rats with myocardial infarction.
作者信息
Li Jin-Yi, Ke Hong-Hong, He Yan, Wen Li-Na, Xu Wei-Yan, Wu Zhi-Fu, Zhao Yan-Mei, Zhong Guo-Qiang
机构信息
Department of Cardiology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China.
出版信息
Cytotechnology. 2018 Feb;70(1):225-234. doi: 10.1007/s10616-017-0136-x. Epub 2017 Sep 19.
At present, little is known about the influence of mesenchymal stem cell (MSC) transplantation on connexin43 (Cx43) and connexin45 (Cx45) remodeling in the ischemic heart. In this study, we investigated the effect of MSC transplantation on Cx43 and Cx45 remodeling in the ischemic heart. Wistar rats were subjected to left anterior descending artery ligation to induce myocardial infarction (MI) and then randomly allocated to receive an intramyocardial injection of PBS (MI group) or 5-azacytidine-induced MSCs (MSCs group). Histological examination and western blotting were performed 4 weeks after cell transplantation. We found that the MSCs exhibited plasticity by differentiating into cardiomyocyte-like cells. Gap junction remodeling after MI was characterized by a decrease in Cx43 expression and an increase in Cx45 expression. MSC transplantation modulated the MI-induced abnormalities by up-regulating Cx43 and down-regulating Cx45 expression. MSCs exhibited plasticity by differentiating into cardiomyocyte-like cells and modulated abnormal Cx43 and Cx45 remodeling following MI.
目前，关于间充质干细胞（MSC）移植对缺血性心脏中连接蛋白43（Cx43）和连接蛋白45（Cx45）重塑的影响知之甚少。在本研究中，我们调查了MSC移植对缺血性心脏中Cx43和Cx45重塑的影响。将Wistar大鼠进行左冠状动脉前降支结扎以诱导心肌梗死（MI），然后随机分为接受心肌内注射PBS的组（MI组）或5-氮杂胞苷诱导的MSC的组（MSC组）。在细胞移植后4周进行组织学检查和蛋白质印迹分析。我们发现MSC通过分化为心肌样细胞表现出可塑性。MI后的缝隙连接重塑的特征是Cx43表达降低和Cx45表达增加。MSC移植通过上调Cx43和下调Cx45表达来调节MI诱导的异常。MSC通过分化为心肌样细胞表现出可塑性，并调节MI后异常的Cx43和Cx45重塑。
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