Mao Rui, Wu Ge, Wang Hui, Lu Pengfei, Li Jun, Li Haitao, Ainiwaer Aimudula, Bai Yiwei, Shu Mingyang, Bao Yongxing, Zhang Wenbao
Department of Radiation Oncology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, 830054, China.
State Key Laboratory Incubation Base for Xinjiang Major Diseases Research and Xinjiang Key Laboratory of Echinococcosis, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, 830054, China.
BMC Infect Dis. 2017 Sep 21;17(1):636. doi: 10.1186/s12879-017-2741-x.
Radiotherapy may represent an alternative treatment modality for cystic echinococcosis (CE), but there is no adequate evidence for it up to now. In this study, we aim to investigate the parasiticidal effects of X-ray on the metacestodes of Echinococcus granulosus in vitro.
Protoscoleces obtained from sheep naturally infected with CE were cultivated in RPMI 1640 medium containing 10% fetal bovine serum (FBS) at 37 °C in 5% CO. Upon encystation on day 14, the metacestodes were subjected to various intensities of X-ray. Metacestode structures were observed using light microscope and transmission electron microscopy (TEM), and Real-Time PCR was carried out to determine the expression of EgTPX, EgHSP70, EgEPC1 and Caspase-3.
On day 14, encystation was noticed in the majority of protoscoleces in the control group. In the X-ray groups, the encystation rate showed significant decrease compared with that of the control group (P < 0.05), especially the groups subjected to a dose of ≥40 Gy (P < 0.01). Light microscope findings indicated the hooklets on the rostellum were deranged in the irradiation group, and malformation was noticed in the suckers in a dose dependent manner. For the TEM findings, the cellular structure of the germinal layer of the cysts was completely interrupted by X-ray on day 7. The expression of EgTPX, EgHSP70, EgEPC1 and Caspase-3 was up-regulated after irradiation, especially at a dose of ≥45Gy (P < 0.05).
X-ray showed parasiticidal effects on the metacestodes of E. granulosus. Irradiation triggered increased expression of EgTPX, EgHSP70, EgEPC1 and Caspase-3.
放射治疗可能是囊性棘球蚴病（CE）的一种替代治疗方式，但目前尚无充分证据支持。在本研究中，我们旨在探讨X射线对体外细粒棘球绦虫原头蚴的杀虫作用。
从自然感染CE的绵羊体内获取原头蚴，在含10%胎牛血清（FBS）的RPMI 1640培养基中，于37℃、5%二氧化碳条件下培养。在第14天包囊形成后，对原头蚴施加不同强度的X射线。使用光学显微镜和透射电子显微镜（TEM）观察原头蚴结构，并进行实时荧光定量PCR以测定EgTPX、EgHSP70、EgEPC1和Caspase-3的表达。
第14天，对照组大多数原头蚴出现包囊形成。在X射线组中，包囊形成率与对照组相比显著降低（P < 0.05），尤其是剂量≥40 Gy的组（P < 0.01）。光学显微镜检查结果表明，照射组头节上的小钩排列紊乱，吸盘出现畸形，且呈剂量依赖性。透射电子显微镜检查结果显示，在第7天，囊肿生发层的细胞结构被X射线完全破坏。照射后EgTPX、EgHSP70、EgEPC1和Caspase-3的表达上调，尤其是剂量≥45 Gy时（P < 0.05）。
X射线对细粒棘球绦虫原头蚴具有杀虫作用。照射引发了EgTPX、EgHSP70、EgEPC1和Caspase-3表达的增加。
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