光动力疗法后人牙龈成纤维细胞碱性成纤维细胞生长因子(bFGF)基因表达分析。
Analysis of gene expression of basic fibroblast growth factor (bFGF) following photodynamic therapy in human gingival fibroblasts.
机构信息
Department of Genetics, Islamic Azad University, Tehran Medical Branch, Theran, Iran.
Laser Research Center of Dentistry (LRCD), Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran; Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
出版信息
Photodiagnosis Photodyn Ther. 2017 Dec;20:144-147. doi: 10.1016/j.pdpdt.2017.09.010. Epub 2017 Sep 19.
BACKGROUND
Periodontal diseases are pathological inflammatory conditions of multifactorial etiology that develops in response to periodontopathic bacteria. Recently, photodynamic therapy (PDT) has begun to be incorporated as an adjuvant treatment for periodontal diseases. However, the effect of PDT on tissue regeneration is not yet completely clear. This study aimed to analyze the gene expression of basic fibroblast growth factor (bFGF) following PDT in human gingival fibroblasts (HuGu) cells in vitro.
MATERIALS AND METHODS
HuGu cells were treated in different groups: 1) ICG (Indocyanine green) alone, 2) Diode laser irradiation alone, 3) PDT (combined ICG with diode laser) and 4) Control (untreated cells). The expression of bFGF gene was evaluated by real-time quantitative reverse transcription PCR (qRT-PCR) in different groups.
RESULTS
ICG-PDT treatment using 1000-2000μg/mL of ICG at fluency of 31.25J/cm displayed a significant dose-dependent increase in expression levels of the bFGF (all P<0.05). The expressions of bFGF were upregulated to 4.13-fold following diode laser irradiation alone with a fluency of 31.25J/cm (P<0.05) while there was no remarkable difference in expression of bFGF following ICG treatment at concentrations of 500-2000μg/mL (all P>0.05).
CONCLUSION
Our results suggest PDT is effective in increasing the expression of bFGF gene, an important factor in periodontal tissue regeneration and could indicate periodontal tissue regeneration.
背景
牙周病是一种多因素病因的病理性炎症性疾病，是对牙周病细菌的反应而发展起来的。最近，光动力疗法（PDT）已开始作为牙周病的辅助治疗方法。然而，PDT 对组织再生的影响尚不完全清楚。本研究旨在分析体外人牙龈成纤维细胞（HuGu）中 PDT 后碱性成纤维细胞生长因子（bFGF）的基因表达。
材料和方法
将 HuGu 细胞分别置于以下各组进行处理：1）单独使用 ICG（吲哚菁绿），2）单独使用二极管激光照射，3）PDT（联合使用 ICG 和二极管激光）和 4）对照组（未处理的细胞）。通过实时定量逆转录 PCR（qRT-PCR）在不同组中评估 bFGF 基因的表达。
结果
ICG-PDT 治疗中，使用浓度为 1000-2000μg/mL、通量为 31.25J/cm 的 ICG 显示出 bFGF 表达水平的显著剂量依赖性增加（均 P<0.05）。单独使用通量为 31.25J/cm 的二极管激光照射可使 bFGF 的表达上调 4.13 倍（P<0.05），而使用浓度为 500-2000μg/mL 的 ICG 处理时 bFGF 的表达无显著差异（均 P>0.05）。
结论
我们的结果表明，PDT 可有效增加 bFGF 基因的表达，bFGF 是牙周组织再生的重要因素，可能提示牙周组织再生。
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