Heidmann Iris, Di Berardino Marco
Acepo, Seyndersloot 20, 1602, HA, Enkhuizen, The Netherlands.
Enza Zaden Research and Development B.V., Enkhuizen, The Netherlands.
Methods Mol Biol. 2017;1669:339-354. doi: 10.1007/978-1-4939-7286-9_25.
Analyzing pollen quality in an efficient and reliable manner is of great importance to the industries involved in seed and fruit production, plant breeding, and plant research. Pollen quality parameters, viability and germination capacity, are analyzed by various staining methods or by in vitro germination assays, respectively. These methods are time-consuming, species-dependent, and require a lab environment. Furthermore, the obtained viability data are often poorly related to in vivo pollen germination and seed set. Here, we describe a quick, label-free method to analyze pollen using microfluidic chips inserted into an impedance flow cytometer (IFC). Using this approach, pollen quality parameters are determined by a single measurement in a species-independent manner. The advantage of this protocol is that pollen viability and germination can be analyzed quickly by a reliable and standardized method.
以高效可靠的方式分析花粉质量对种子和果实生产、植物育种及植物研究等行业至关重要。花粉质量参数，即活力和萌发能力，分别通过各种染色方法或体外萌发试验进行分析。这些方法耗时、依赖物种且需要实验室环境。此外，所获得的活力数据往往与体内花粉萌发和结实率相关性较差。在此，我们描述了一种使用插入阻抗流式细胞仪（IFC）的微流控芯片快速、无标记分析花粉的方法。使用这种方法，花粉质量参数可通过单一测量以不依赖物种的方式确定。该方案的优点是可以通过可靠且标准化的方法快速分析花粉活力和萌发情况。
