Li Yuan, Wang Lian-Xin, Xie Yan-Ming
Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2016 Dec;41(24):4521-4532. doi: 10.4268/cjcmm20162408.
Biomolecular network analysis was used to predict the mechanism of Salvianolate injection combined with aspirin for the treatment of stable angina pectoris(SAP). Related genes of Salvianolate injection, aspirin and SAP were obtained from Genecards, STITCH and DisGeNET databases. Agilent literature search software was used to construct biomolecular network; modules were identified by AP, MCODE and MCL methods. DAVID software was used for identification of related KEGG pathways. Results showed that Salvianolate injection and aspirin had a coverage rate of 45.92%, 62.56% respectively for SAP molecular network, and the coverage rate was 71.64% in combined use. The top 10 important nodes of SAP overlapped with Salvianolate injection and aspirin included MAPK14, MAPK8, IL-6 and IL-8. The important SAP nodes overlapped with Salvianolate injection alone included AKT1 and IFNG, and the important SAP nodes overlapped with aspirin included EPHB2 and TP53. Related SAP signaling pathways with combined Salvianolate injection and aspirin included Jak-STAT signaling pathway and MAPK signaling pathway. Related SAP signaling pathways with Salvianolate injection alone included VEGF signaling pathway and type 1 diabetes signaling pathway. Related SAP signaling pathways with aspirin alone included AA metabolism, linoleic acid metabolism signaling pathway, etc. The results showed that Salvianolate injection and aspirin combination had an enhancement effect in treatment of SAP through anti-inflammatory reaction and inhibition of atherosclerosis development; in addition, the combination use may have an additive effect through the antiplatelet aggregation, protecting endothelial cells, regulating blood lipid and regulating glucose metabolism.
采用生物分子网络分析预测丹参多酚酸盐注射液联合阿司匹林治疗稳定型心绞痛（SAP）的作用机制。从Genecards、STITCH和DisGeNET数据库获取丹参多酚酸盐注射液、阿司匹林和SAP的相关基因。使用安捷伦文献检索软件构建生物分子网络；通过AP、MCODE和MCL方法识别模块。使用DAVID软件识别相关的KEGG通路。结果显示，丹参多酚酸盐注射液和阿司匹林对SAP分子网络的覆盖率分别为45.92%、62.56%，联合使用时覆盖率为71.64%。与丹参多酚酸盐注射液和阿司匹林重叠的SAP前10个重要节点包括MAPK14、MAPK8、IL-6和IL-8。单独与丹参多酚酸盐注射液重叠的重要SAP节点包括AKT1和IFNG，与阿司匹林重叠的重要SAP节点包括EPHB2和TP53。丹参多酚酸盐注射液与阿司匹林联合相关的SAP信号通路包括Jak-STAT信号通路和MAPK信号通路。单独与丹参多酚酸盐注射液相关的SAP信号通路包括VEGF信号通路和1型糖尿病信号通路。单独与阿司匹林相关的SAP信号通路包括AA代谢、亚油酸代谢信号通路等。结果表明，丹参多酚酸盐注射液与阿司匹林联合应用通过抗炎反应和抑制动脉粥样硬化发展对SAP治疗有增强作用；此外，联合使用可能通过抗血小板聚集、保护内皮细胞、调节血脂和调节糖代谢产生相加效应。
