Huang Xue-Ying, Fan Kai, Ye Yan-Fang, Wang Bin, Wu Wei-Ren, Lan Tao
Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Yi Chuan. 2017 Sep 20;39(9):856-862. doi: 10.16288/j.yczz.17-192.
We explored the practical effect of the genetic analysis of simple sequence length polymorphism (SSLP) molecular markers in rice in the genetics lab course. Two parents and their F population were analyzed and detected with three SSLP molecular markers that located on two chromosomes of the rice genome. The markers' genotype data were used to verify the three laws of genetics, including segregation, independent assortment and linkage and crossing-over. Our practice has proved not only beneficial to deepen students' understandings about the three laws of genetics, but also conducive to cultivate students' interests in research and innovation and improve their skills and comprehensive analysis abilities. At the same time, the application scope of the experiment was discussed. This comprehensive experiment is also useful for the transformation of scientific research achievements into undergraduate experimental teaching.
我们在遗传学实验课程中探索了简单序列长度多态性(SSLP)分子标记的遗传分析在水稻中的实际效果。对两个亲本及其F群体进行了分析,并使用位于水稻基因组两条染色体上的三个SSLP分子标记进行检测。标记的基因型数据用于验证遗传学的三大定律,包括分离定律、自由组合定律以及连锁与交换定律。我们的实践证明,这不仅有利于加深学生对遗传学三大定律的理解,还有助于培养学生的科研和创新兴趣,提高他们的技能和综合分析能力。同时,还讨论了该实验的应用范围。这个综合性实验对于将科研成果转化为本科实验教学也很有用。