Gao Mingchun, Liu Ying, Guo Yongli, Wang Yujiao, Dai Haiyue, Song Zhifeng, Wang Junwei, Han Wenyu
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, PR China.
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, PR China.
Dev Comp Immunol. 2018 Jan;78:91-99. doi: 10.1016/j.dci.2017.09.016. Epub 2017 Sep 21.
The multigene family of rabbit IFN-α (RbIFN-α) is located on chromosome 1, which shows seven functional genes in type I IFN locus. A novel RbIFN-α that remains unlocated in the rabbit genome was amplified and designated as the first novel rabbit IFN-α (RbIFN-αNov1), which possesses the typical molecular characteristics of type I IFNs and could be induced in RK-13 cells and peripheral blood mononuclear cells. After the mature peptide of RbIFN-αNov1 was expressed, its antiviral activity, physicochemical characteristics, and cytotoxicity were determined in vitro. Results indicated that RbIFN-αNov1 exerted a high specific antiviral activity against VSV and a low cytotoxic effect on RK-13 cells. RbIFN-αNov1 showed high sensitivity to trypsin and remained relatively stable after acid, alkali, or heat treatment. RbIFN-αNov1 could induce Mx1 expression on RK-13 cells and activate the NF-κB, ISRE and BoIFN-β promoter activities on bovine testicular cells. Overall, our research on RbIFN-αNov1 not only enriches the knowledge about rabbit IFNs but also makes RbIFN-αNov1 have the potential to be used as an effective therapeutic agent for rabbit viral diseases.
兔α干扰素（RbIFN-α）多基因家族位于1号染色体上，在I型干扰素基因座中显示有7个功能基因。扩增出一个在兔基因组中尚未定位的新型RbIFN-α，并将其命名为首个新型兔干扰素α（RbIFN-αNov1），它具有I型干扰素的典型分子特征，可在RK-13细胞和外周血单个核细胞中诱导产生。RbIFN-αNov1成熟肽表达后，对其体外抗病毒活性、理化特性及细胞毒性进行了测定。结果表明，RbIFN-αNov1对水疱性口炎病毒（VSV）具有高特异性抗病毒活性，对RK-13细胞的细胞毒性作用较低。RbIFN-αNov1对胰蛋白酶高度敏感，经酸、碱或热处理后相对稳定。RbIFN-αNov1可诱导RK-13细胞上Mx1的表达，并激活牛睾丸细胞上的NF-κB、ISRE和牛β干扰素（BoIFN-β）启动子活性。总体而言，我们对RbIFN-αNov1的研究不仅丰富了关于兔干扰素的知识，而且使RbIFN-αNov1有潜力用作兔病毒性疾病的有效治疗剂。
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