Campuzano-Caballero Juan Carlos, Uribe Mari Carmen
Laboratorio de Biología de la Reproducción Animal. Departamento de Biología Comparada. Facultad de Ciencias, Universidad Nacional Autónoma de México, Av. Universidad 3000, Delegación Coyoacán, C. P. 04510, Ciudad Universitaria, Ciudad de México, México.
J Morphol. 2017 Dec;278(12):1647-1655. doi: 10.1002/jmor.20738. Epub 2017 Sep 25.
Female teleosts do not develop Müllerian ducts; consequently, the ovary of teleosts contains two zones: germinal and gonoduct. The gonoduct lacks germinal cells, but has relevant functions in the reproductive process. We describe the functional morphology of the gonoduct in the viviparous teleost Poeciliopsis gracilis during nongestation and gestation stages. This study tests the hypothesis that the gonoduct functions as a barrier between the germinal zone and the exterior. By providing information about morphology and function of the gonoduct we show that this part of the ovary has an essential role in the reproduction of teleosts. The ovaries were processed by histological technique and stained with hematoxylin-eosin (H-E), Masson's trichrome, toluidine blue and periodic acid-Schiff (PAS). The gonoduct is divided into three regions: cephalic, middle, and caudal. In the cephalic and middle regions there are mucosal folds that extend into the gonoductal lumen, forming structures similar to a cervix. The caudal region has two portions: the anterior contains a dorsal invagination and exocrine glands among columnar cells; the posterior has a ventral flexion and stratified epithelium with apical secretory cells. The morphology of this epithelium indicates two functions: (a) secretory by the apical columnar cells, and (b) protection through the stratification. Another peculiarity of the caudal region is that both ducts, reproductive and digestive, converge in a common cavity at their caudal ends, forming a cloacal region. The histology of the gonoduct indicates relevant functions including: (1) the control of the luminal diameter by the muscle and the presence of mucosal folds, like a cervix; (2) the relationship with the spermatozoa during insemination and storing them in mucosal folds; (3) the support of immunological processes; (4) secretory activities; (5) forming the duct during birth; and (6) possibly, acts as a barrier against parasite infestations.
雌性硬骨鱼没有缪勒氏管；因此，硬骨鱼的卵巢包含两个区域：生殖区和生殖导管区。生殖导管区没有生殖细胞，但在生殖过程中具有相关功能。我们描述了胎生硬骨鱼细纹食蚊鱼在非妊娠期和妊娠期生殖导管的功能形态。本研究检验了这样一个假设，即生殖导管起到生殖区与外部之间屏障的作用。通过提供有关生殖导管形态和功能的信息，我们表明卵巢的这一部分在硬骨鱼的繁殖中起着至关重要的作用。卵巢采用组织学技术处理，并用苏木精 - 伊红（H - E）、马松三色染色法、甲苯胺蓝和过碘酸 - 希夫（PAS）染色。生殖导管分为三个区域：头部、中部和尾部。在头部和中部区域有黏膜褶皱延伸到生殖导管腔内，形成类似于子宫颈的结构。尾部区域有两部分：前部包含一个背侧内陷和柱状细胞之间的外分泌腺；后部有一个腹侧弯曲和具有顶端分泌细胞的复层上皮。这种上皮的形态表明有两种功能：（a）顶端柱状细胞的分泌功能，以及（b）通过分层实现的保护功能。尾部区域的另一个特点是，生殖导管和消化导管在其尾端汇合于一个共同的腔室，形成泄殖腔区域。生殖导管的组织学表明其具有多种相关功能，包括：（1）通过肌肉和黏膜褶皱的存在来控制管腔直径，类似于子宫颈；（2）在受精过程中与精子的相互作用以及将精子储存在黏膜褶皱中；（3）支持免疫过程；（4）分泌活动；（5）在分娩时形成导管；以及（6）可能作为抵御寄生虫感染的屏障。
