Hozumi Shunya, Aoki Shun, Kikuchi Yutaka
Department of Biological Science, Graduate School of Science, Hiroshima University, Kagamiyama 1-3-1, Higashi-Hiroshima, Hiroshima, 739-8526 Japan.
Department of Biological Science, Graduate School of Science, Hiroshima University, Kagamiyama 1-3-1, Higashi-Hiroshima, Hiroshima, 739-8526 Japan
Development. 2017 Nov 1;144(21):4015-4025. doi: 10.1242/dev.151746. Epub 2017 Sep 25.
Asymmetric nuclear positioning is observed during animal development, but its regulation and significance in cell differentiation remain poorly understood. Using zebrafish blastulae, we provide evidence that nuclear movement towards the yolk syncytial layer, which comprises extraembryonic tissue, occurs in the first cells fated to differentiate into the endoderm. Nodal signaling is essential for nuclear movement, whereas nuclear envelope proteins are involved in movement through microtubule formation. Positioning of the microtubule-organizing center, which is proposed to be crucial for nuclear movement, is regulated by Nodal signaling and nuclear envelope proteins. The non-Smad JNK signaling pathway, which is downstream of Nodal signaling, regulates nuclear movement independently of the Smad pathway, and this nuclear movement is associated with Smad signal transduction toward the nucleus. Our study provides insight into the function of nuclear movement in Smad signaling toward the nucleus, and could be applied to the control of TGFβ signaling.
在动物发育过程中观察到不对称核定位，但其在细胞分化中的调控及意义仍知之甚少。利用斑马鱼囊胚，我们提供了证据表明，核向包含胚外组织的卵黄合胞体层移动，发生在最早注定分化为内胚层的细胞中。Nodal信号对于核移动至关重要，而核膜蛋白通过微管形成参与移动。微管组织中心的定位被认为对核移动至关重要，它受Nodal信号和核膜蛋白调控。作为Nodal信号下游的非Smad JNK信号通路，独立于Smad通路调控核移动，且这种核移动与Smad信号向细胞核的转导相关。我们的研究深入了解了核移动在Smad信号向细胞核转导中的功能，并可应用于TGFβ信号的控制。
