Tsuji Sayaka, Ichihashi Norikazu
Department of Bioinformatics Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565-0871, Japan.
Graduate School of Frontier Biosciences, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565-0871, Japan.
Biochem Biophys Rep. 2017 May 12;10:325-328. doi: 10.1016/j.bbrep.2017.05.002. eCollection 2017 Jul.
Ribosome composition, consisting of rRNA and ribosomal proteins, is highly conserved among a broad range of organisms. However, biochemical studies focusing on ribosomal subunit exchangeability between organisms remain limited. In this study, we show that chimeric ribosomes, composed of and or and subunits, are active for β-galactosidase translation in a highly purified translation system. Activities of the chimeric ribosomes showed only a modest decrease when using 30 S subunits, indicating functional conservation of the 50 S subunit between these bacterial species.
核糖体由rRNA和核糖体蛋白组成,在广泛的生物体中高度保守。然而,针对生物体之间核糖体亚基可交换性的生化研究仍然有限。在本研究中,我们表明,由 和 或 和 亚基组成的嵌合核糖体在高度纯化的 翻译系统中对β-半乳糖苷酶翻译具有活性。当使用 30 S亚基时,嵌合核糖体的活性仅略有下降,表明这些细菌物种之间50 S亚基的功能保守性。