小鼠表皮片中非特异性酯酶反应性Ia抗原和Thy-1抗原的顺序显示

The sequential demonstration of non-specific esterase reactivity Ia antigen and Thy-1 antigen in murine epidermal sheets.

作者信息

Howells G L, Williams D M

机构信息

Department of Oral Pathology, London Hospital Medical College, England, U.K.

出版信息

Arch Oral Biol. 1987;32(9):679-83. doi: 10.1016/0003-9969(87)90045-8.

DOI:10.1016/0003-9969(87)90045-8

PMID:2895629

Abstract

Immunofluorescence demonstrating Ia and Thy-1 antigens and non-specific esterase (NSE) enzyme histochemistry were performed sequentially on sheets of ear epidermis from CBA, C3H.OH, A/J, and Balb/c mice. The same areas of epidermis were photographed after each reaction and individual cells identified and compared. Thy-1+ dendritic cells expressed neither Ia antigen nor NSE reactivity. No cell was found to express Ia antigen or NSE alone: thus all Langerhans cells (LC) in normal murine epidermis appeared to co-express Ia antigen and NSE reactivity. LC expressing greatly increased amounts of Ia antigen were occasionally seen apposed to Thy-1+ cells suggesting that these cells may be immunologically active--perhaps involved in antigen presentation.

摘要

对CBA、C3H.OH、A/J和Balb/c小鼠耳部表皮片依次进行免疫荧光检测Ia和Thy-1抗原，以及非特异性酯酶（NSE）酶组织化学检测。每次反应后对表皮的相同区域进行拍照，并对单个细胞进行识别和比较。Thy-1 +树突状细胞既不表达Ia抗原，也不具有NSE反应性。未发现单独表达Ia抗原或NSE的细胞：因此，正常小鼠表皮中的所有朗格汉斯细胞（LC）似乎都共表达Ia抗原和NSE反应性。偶尔可见与Thy-1 +细胞相邻的Ia抗原表达量大幅增加的LC，提示这些细胞可能具有免疫活性——或许参与抗原呈递。