利用 xTAG 液相芯片技术在急性视网膜坏死患者眼内检测疱疹病毒。
Intraocular Detection of Herpes viruses by xTAG Liquid Chip Technology in Patients with Acute Retinal Necrosis.
机构信息
a Ophthalmology Department, Peking Union Medical College Hospital , Chinese Academy of Medical Sciences , Beijing , China.
b Department of Clinical Laboratory, Peking Union Medical College Hospital , Chinese Academy of Medical Sciences , Beijing , China.
出版信息
Ocul Immunol Inflamm. 2018;26(8):1271-1277. doi: 10.1080/09273948.2017.1347266. Epub 2017 Sep 29.
PURPOSE
To evaluate the performance of the xTAG liquid chip technology (xTAG-LCT) for etiological diagnosis of acute retinal necrosis (ARN).
METHODS
Fifteen vitreous and 3 aqueous samples from 18 ARN patients were analyzed by xTAG-LCT and multiplex PCR (mPCR)/quantitative PCR (qPCR).
RESULTS
xTAG-LCT revealed positive results in 17 of the 18 samples: 10 for Varicella Zoster Virus (VZV) alone; 5 for VZV and Epstein-Barr virus (EBV); 1 for herpes simplex viruses type 1 (HSV-1) and EBV; 1 for VZV, HSV-1 and EBV. While mPCR revealed the same results as xTAG-LCT for VZV and HSV-1 in all samples, only 2 of the 7 samples positive for EBV on xTAG-LCT were confirmed by qPCR. None of the 28 control vitreous samples from 8 non-ARN patients and 10 pair of cadaveric eyes was positive for any of the tested viruses.
CONCLUSIONS
xTAG-LCT could be a useful alternative for etiological diagnosis of ARN.
目的
评估 xTAG 液相芯片技术（xTAG-LCT）在急性视网膜坏死（ARN）病因诊断中的性能。
方法
对 18 例 ARN 患者的 15 份玻璃体和 3 份房水样本进行了 xTAG-LCT 和多重聚合酶链反应（mPCR）/定量聚合酶链反应（qPCR）分析。
结果
xTAG-LCT 在 18 例样本中的 17 例中显示出阳性结果：单纯水痘带状疱疹病毒（VZV）10 例；VZV 和 EBV 5 例；单纯单纯疱疹病毒 1 型（HSV-1）和 EBV 1 例；VZV、HSV-1 和 EBV 各 1 例。虽然 mPCR 在所有样本中均与 xTAG-LCT 对 VZV 和 HSV-1 显示出相同的结果，但 xTAG-LCT 对 EBV 呈阳性的 7 个样本中，仅通过 qPCR 证实了 2 个样本。8 例非 ARN 患者和 10 对尸眼的 28 例对照玻璃体样本均未对任何检测到的病毒呈阳性。
结论
xTAG-LCT 可能是 ARN 病因诊断的一种有用替代方法。
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