Effects of rituximab and dexamethasone on regulatory and proinflammatory B-cell subsets in patients with primary immune thrombocytopenia.

OBJECTIVE

To investigate the cytokine production and surface marker composition of B cells in adult patients with newly diagnosed primary immune thrombocytopenia (ITP) before and 12 months after treatment with rituximab + dexamethasone (RTX+DXM) or dexamethasone (DXM).

METHODS

Peripheral blood mononuclear cells were isolated from nine patients treated with RTX+DXM, seven patients treated with DXM, and seven healthy donors. Expression of the cell-surface markers CD5, CD27, CD25, and CD19, and intracellular content of IL-6 and IL-10 were measured by flow cytometry.

RESULTS

PBMCs from ITP patients at baseline contained a lower proportion of IL-10 B cells (P < .01) and IL-6 B cells (P < .01) than healthy controls. All patients responded to therapy and levels were normalized at 12 months. The proportion of CD5 B cells increased (P < .01) and CD27 memory B cells decreased (P < .05) 12 months after treatment with RTX+DXM compared to baseline, with an inverse correlation between platelet numbers and the proportion of CD27 B cells (R = -0.71; P < .05).

CONCLUSION

Both treatment regimens normalized the frequencies of cytokine-producing B cells. The additional increase in CD5 B cells after RTX+DXM is compatible with induction of Bregs.