Wei Xiaopeng, Mao Linchun, Han Xueyuan, Lu Wenjing, Xie Dandan, Ren Xingchen, Zhao Yuying
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China.
Zhejiang Key Laboratory of Agro-Food Processing, Zhejiang R&D Center of Food Technology and Equipment, Fuli Institute of Food Science, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China.
J Sci Food Agric. 2018 Apr;98(6):2223-2230. doi: 10.1002/jsfa.8709. Epub 2017 Dec 14.
Rapid wound healing would be critical for successful long-term storage of fruits and vegetables. However, there was no direct evidence for the requirement and efficiency of oxygen in the fruit wound-healing process. This study was conducted to investigate the role of oxygen in wound-induced suberization by analyzing melanin, suberin polyphenolics (SPPs) and related enzymes in half-cut kiwifruits exposed to 100%, 50%, 21% and 0% oxygen.
By 3 days after wounding, the wound surface of kiwifruit in high (50 and 100%) oxygen appeared as a continuous layer of melanin and SPPs underneath, which effectively prevent excessive water vapor loss from the fruit halves. In contrast, melanin and SPPs deposition in the wound surface in 0% oxygen was significantly reduced, with high water vapor loss. Rapid decrease of soluble phenolic acids (caffeic, p-coumaric, ferulic acids) was coupled with the increase of bound ferulic acid (coniferyl diacetate) especially in high oxygen by 9 days after wounding. Meanwhile, high oxygen enhanced peroxidase, catalase, phenylalanine ammonia-lyase, and polyphenol oxidase activities.
Oxygen is required for wound-induced melanin and SPPs formation, and high oxygen is effective in promoting wound suberization in postharvest kiwifruit. © 2017 Society of Chemical Industry.
快速伤口愈合对于水果和蔬菜的长期成功储存至关重要。然而，在水果伤口愈合过程中，对于氧气的需求和效率尚无直接证据。本研究通过分析暴露于100%、50%、21%和0%氧气环境下的半切猕猴桃中黑色素、木栓质多酚（SPPs）及相关酶，来探究氧气在伤口诱导木栓化中的作用。
受伤3天后，处于高（50%和100%）氧环境下的猕猴桃伤口表面出现一层连续的黑色素层，其下为SPPs，这有效地防止了半个果实过多的水分散失。相比之下，处于0%氧气环境下的伤口表面黑色素和SPPs沉积显著减少，水分散失量大。受伤9天后，可溶性酚酸（咖啡酸、对香豆酸、阿魏酸）迅速减少，尤其是在高氧环境下，结合型阿魏酸（松柏基二乙酸酯）增加。同时，高氧增强了过氧化物酶、过氧化氢酶、苯丙氨酸解氨酶和多酚氧化酶的活性。
伤口诱导的黑色素和SPPs形成需要氧气，高氧可有效促进采后猕猴桃伤口木栓化。©2017化学工业协会。
