Zhang Xiao-Ying, Lü Fa-Qin, Tang Jie
Department of Ultrasonography, Aviation General Hospital, Beijing 100012, China.
Department of Ultrasonography, General Hospital of PLA, Beijing 100853, China.
Zhonghua Nan Ke Xue. 2016 Jul;22(7):596-601.
To explore the effects of ischemic preconditioning on the level of serum testosterone (T) and apoptosis of spermatogenic cells in rabbits with testicular ischemia-reperfusion injury induced by testicular torsion.
A total of 15 New Zealand male rabbits were randomly divided into groups A (control), B (ischemia-reperfusion), and C (ischemic preconditioning). The animals of group A were subjected to exposure of the right spermatic cord without ischemia, those of group B received 60-minute non-invasive occlusion of the right spermatic cord followed by 3 days of reperfusion, and those of group C underwent 5-minute occlusion plus 5-minute reperfusion of the right spermatic cord followed by the same procedure as that for group B. Then the rabbits were narcotized with 3% barbital sodium, the whole blood collected for examination of the serum T content and the testis tissues obtained from both the ischemic and healthy sides for HE and TUNEL staining.
After operation, the body weight was significantly increased as compared with the baseline in groups A (［2.65±0.07］ vs ［2.45±0.07］ kg, P<0.05) and C (［3.03±0.11］ vs ［2.92±0.07］ kg, P<0.05), but not in group B (［3.05±0.07］ vs ［3.05±0.07］ kg, P>0.05). The serum T level showed no statistically significant difference in group A before and after operation (［139.59±9.39］ vs ［140.19±9.47］ ng/L, P>0.05), but was remarkably lower after operation than the baseline in groups B ［148.06±3.31］ vs ［74.12±4.00］ ng/L, P<0.01) and C (［133.75±6.48］ vs［94.76±3.13］ ng/L, P<0.01) as well as than the postoperative index in group A (P<0.01). In comparison with group A and the healthy side of group B, the testis tissue of the ischemic side in group B exhibited structural damage of most of the seminiferous tubules with disappearance of spermatogenic cell structures, apoptosis of spermatogenic cells, and exudation of light-eosin edema fluid in the mesenchyme and lumen, with a markedly increased apoptosis index (P<0.01) and a significantly decreased Johsen's score (P<0.01). Compared with ischemic side of group B, The testis tissue of the ischemic side in group C was restored to normal as compared with that in group B, with a dramatically decreased apoptosis index (P<0.01) and a remarkably increased Johnsen's score (P<0.01).
Ischemic preconditioning can raise the decreased serum T level and reduce the apoptosis of spermatogenic cells in rabbits with testicular ischemia-reperfusion injury, which could be applied as a potential option for the clinical treatment of testicular ischemia-reperfusion injury.
探讨缺血预处理对睾丸扭转所致睾丸缺血再灌注损伤家兔血清睾酮（T）水平及生精细胞凋亡的影响。
将15只新西兰雄性家兔随机分为A组（对照组）、B组（缺血再灌注组）和C组（缺血预处理组）。A组家兔仅暴露右侧精索，不进行缺血处理；B组家兔对右侧精索进行60分钟无创阻断，随后再灌注3天；C组家兔对右侧精索进行5分钟阻断加5分钟再灌注，随后进行与B组相同的处理。然后用3%戊巴比妥钠麻醉家兔，采集全血检测血清T含量，并取缺血侧和健康侧睾丸组织进行苏木精-伊红（HE）染色和末端脱氧核苷酸转移酶介导的缺口末端标记（TUNEL）染色。
术后，A组（［2.65±0.07］ vs ［2.45±0.07］ kg，P<0.05）和C组（［3.03±0.11］ vs ［2.92±0.07］ kg，P<0.05）家兔体重较基线显著增加，而B组（［3.05±0.07］ vs ［3.05±0.07］ kg，P>0.05）无明显变化。A组术后血清T水平术前术后差异无统计学意义（［139.59±9.39］ vs ［140.19±9.47］ ng/L，P>0.05），但B组（［148.06±3.31］ vs ［74.12±4.00］ ng/L，P<0.01）和C组（［133.75±6.48］ vs［94.76±3.13］ ng/L，P<0.01）术后血清T水平较基线显著降低，且低于A组术后指标（P<0.01）。与A组及B组健康侧相比，B组缺血侧睾丸组织大部分生精小管结构破坏，生精细胞结构消失，生精细胞凋亡，间质和管腔内有淡嗜酸性水肿液渗出，凋亡指数显著升高（P<0.01），Johnsen评分显著降低（P<0.01）。与B组缺血侧相比，C组缺血侧睾丸组织与B组相比恢复正常，凋亡指数显著降低（P<0.01），Johnsen评分显著升高（P<0.01）。
缺血预处理可提高睾丸缺血再灌注损伤家兔降低的血清T水平，减少生精细胞凋亡，可作为睾丸缺血再灌注损伤临床治疗的一种潜在选择。
