猪源人ABO血型直系同源基因全序列信息的测定及血型频率的品种差异

Determination of complete sequence information of the human ABO blood group orthologous gene in pigs and breed difference in blood type frequencies.

作者信息

Choi Min-Kyeung, Le Minh Thong, Cho Hyesun, Yum Joori, Kang Mingue, Song Hyuk, Kim Jin-Hoi, Chung Hak Jae, Hong Kwonho, Park Chankyu

机构信息

Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul, South Korea.

Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul, South Korea; Humanized Pig Research Center (SRC), 120 Neungdong-ro, Gwangjin-gu, Seoul, South Korea.

出版信息

Gene. 2018 Jan 15;640:1-5. doi: 10.1016/j.gene.2017.09.047. Epub 2017 Sep 29.

DOI:10.1016/j.gene.2017.09.047

PMID:28966132

Abstract

The sequence information of the genomic form of the human ABO blood group orthologous gene (erythrocyte antigen A, EAA) is not complete in pigs. Therefore, we cloned and characterized the nucleotide sequence of EAA intron 7, which is critical to understand genetic difference between A and 0 blood groups in pigs, covering complete genomic sequence information of EAA excluding a ~560bp unsequencible gap. We also analyzed genetic polymorphisms within EAA intron 7 and exon 8. We found difference in A0 blood group frequencies among pig breeds. In addition, we designed a new genomic DNA-based A0 blood group typing method and improved the accuracy and simplicity of the typing.

摘要

猪的人类ABO血型直系同源基因（红细胞抗原A，EAA）基因组形式的序列信息并不完整。因此，我们克隆并鉴定了EAA内含子7的核苷酸序列，该序列对于理解猪A和O血型之间的遗传差异至关重要，涵盖了EAA完整的基因组序列信息，但不包括约560bp无法测序的缺口。我们还分析了EAA内含子7和外显子8内的遗传多态性。我们发现猪品种间A0血型频率存在差异。此外，我们设计了一种基于基因组DNA的新型A0血型分型方法，提高了分型的准确性和简便性。