Bai Yunlong, Zhang Liangshuan, Jiang Yanan, Ju Jiaming, Li Guiyang, Xu Juan, Jiang Xing, Zhang Peng, Lang Linchuan, Sadkovaya Olga, Glybochko Peter V, Zhang Wei, Yang Baofeng
Department of Pharmacology, State Province Key Laboratories of Biomedicine and Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education, College of Pharmacy, Harbin Medical University, Harbin, People's Republic of China.
Department of Pharmacology, State Province Key Laboratories of Biomedicine and Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education, College of Pharmacy, Harbin Medical University, Harbin, People's Republic of China.
Sex Med. 2017 Dec;5(4):e261-e271. doi: 10.1016/j.esxm.2017.06.006. Epub 2017 Sep 29.
Obesity is a potential risk factor for erectile dysfunction (ED). MicroRNAs (miRNAs) regulate the expression of genes involved in various pathophysiologic processes.
To identify the miRNA profile in the corpus cavernosum (CC) of obese rats with ED and elucidate the potential function of miRNA in the pathogenesis of ED.
Obesity was induced in rats by a high-fat diet. After the erectile function test, experimental animals were divided into two groups: obese rats with ED and obese rats with normal erectile function. The CCs from these rats were collected for miRNA microarray analysis. The results were verified by real-time polymerase chain reaction analysis. Subsequently, the targets of differentially expressed miRNAs were predicted. Bioinformatics analysis was applied to predict the functions of differentially expressed miRNAs in ED. Apomorphine-induced penile erection and intracavernous pressure measurements were used to evaluate the effects of miRNA on the erectile function of rats.
MiRNA expression in the CC of obese rats with ED and those with normal erectile function was detected by miRNA microarray analysis. Candidate miRNAs were validated by real-time polymerase chain reaction. Bioinformatics analysis was used to predict the functions of miRNAs. Apomorphine-induced penile erection and intracavernous pressure measurements were used to reflect the erectile function of rats.
Sixty-eight miRNAs were differentially expressed in the CC of obese rats with ED (≥1.5-fold change). The real-time polymerase chain reaction results were consistent with the miRNA microarray analysis results. Specifically, miR-328a was significantly upregulated in rats with ED compared with control rats and was chosen for functional evaluation in the pathogenesis of ED. Overexpression of miR-328a noticeably decreased the erectile response to apomorphine and the expression of heme oxygenase-1.
MiRNAs are involved in the pathogenesis of obesity-related ED. MiR-328a might facilitate the induction of ED. Bai Y, Zhang L, Jiang Y, et al. Identification and Functional Verification of MicroRNAs in the Obese Rat With Erectile Dysfunction. Sex Med 2017;5:e261-e271.
肥胖是勃起功能障碍（ED）的一个潜在风险因素。微小RNA（miRNA）可调节参与各种病理生理过程的基因表达。
鉴定患有ED的肥胖大鼠海绵体（CC）中的miRNA谱，并阐明miRNA在ED发病机制中的潜在作用。
通过高脂饮食诱导大鼠肥胖。在进行勃起功能测试后，将实验动物分为两组：患有ED的肥胖大鼠和勃起功能正常的肥胖大鼠。收集这些大鼠的CC进行miRNA微阵列分析。结果通过实时聚合酶链反应分析进行验证。随后，预测差异表达miRNA的靶标。应用生物信息学分析预测差异表达miRNA在ED中的功能。使用阿扑吗啡诱导的阴茎勃起和海绵体内压力测量来评估miRNA对大鼠勃起功能的影响。
通过miRNA微阵列分析检测患有ED的肥胖大鼠和勃起功能正常的肥胖大鼠CC中的miRNA表达。通过实时聚合酶链反应验证候选miRNA。使用生物信息学分析预测miRNA的功能。使用阿扑吗啡诱导的阴茎勃起和海绵体内压力测量来反映大鼠的勃起功能。
在患有ED的肥胖大鼠的CC中，有68种miRNA差异表达（变化倍数≥1.5倍）。实时聚合酶链反应结果与miRNA微阵列分析结果一致。具体而言，与对照大鼠相比，miR-328a在患有ED的大鼠中显著上调，并被选择用于ED发病机制的功能评估。miR-328a的过表达显著降低了对阿扑吗啡的勃起反应以及血红素加氧酶-1的表达。
miRNA参与肥胖相关ED的发病机制。miR-328a可能促进ED的发生。白Y，张L，姜Y等。勃起功能障碍肥胖大鼠中微小RNA的鉴定与功能验证。性医学2017；5：e261-e271。
