纳米串nCounter®技术在乳腺癌中的应用：与定量实时聚合酶链反应、杂交及免疫组织化学的比较分析

NanoString nCounter® Approach in Breast Cancer: A Comparative Analysis with Quantitative Real-Time Polymerase Chain Reaction, Hybridization, and Immunohistochemistry.

作者信息

Hyeon Jiyeon, Cho Soo Youn, Hong Min Eui, Kang So Young, Do Ingu, Im Young Hyuck, Cho Eun Yoon

机构信息

Department of Pathology and Translational Genomics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.

Department of Pathology, Gangnam Sacred Heart Hospital, Hallym University College of Medicine, Seoul, Korea.

出版信息

J Breast Cancer. 2017 Sep;20(3):286-296. doi: 10.4048/jbc.2017.20.3.286. Epub 2017 Sep 22.

DOI:10.4048/jbc.2017.20.3.286

PMID:28970855

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5620444/

Abstract

PURPOSE

Accurate testing for estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) is essential for breast cancer treatment. At present, immunohistochemistry (IHC)/florescence hybridization (FISH) are widely accepted as the standard testing methods. To investigate the value of NanoString nCounter®, we performed its comparative analysis with IHC/FISH and real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) for the assessment of ER, PR, and HER2.

METHODS

Data on IHC/FISH results for ER, PR, and HER2 in 240 patients from a single tertiary hospital in Korea were collected and compared with NanoString nCounter® and qRT-PCR results at a single institution.

RESULTS

Expression levels for each gene using NanoString nCounter® showed good correlation with the corresponding data for protein expression by IHC (<0.001) and gene amplification status for (<0.001). Comparisons between gene expression and IHC data showed good overall agreement with a high area under the curve (AUC) for /ER (AUC=0.939), /PR (AUC=0.796), and /HER2 (AUC=0.989) (<0.001).

CONCLUSION

The quantification of , , and mRNA expression with NanoString nCounter® may be a viable alternative to conventional IHC/FISH methods.

摘要

目的

准确检测雌激素受体（ER）、孕激素受体（PR）和人表皮生长因子受体2（HER2）对于乳腺癌治疗至关重要。目前，免疫组织化学（IHC）/荧光杂交（FISH）被广泛认可为标准检测方法。为了研究NanoString nCounter®的价值，我们将其与IHC/FISH以及实时定量逆转录聚合酶链反应（qRT-PCR）进行了比较分析，以评估ER、PR和HER2。

方法

收集了韩国一家三级医院240例患者的ER、PR和HER2的IHC/FISH结果数据，并与同一机构的NanoString nCounter®和qRT-PCR结果进行比较。

结果

使用NanoString nCounter®检测的每个基因的表达水平与IHC检测的蛋白质表达相应数据（<0.001）以及基因扩增状态（<0.001）显示出良好的相关性。基因表达与IHC数据之间的比较显示总体一致性良好，/ER（AUC = 0.939）、/PR（AUC = 0.796）和/HER2（AUC = 0.989）的曲线下面积（AUC）较高（<0.001）。