未从耳硬化症患者镫骨中检测到麻疹病毒。

Absence of Measles Virus Detection from Stapes of Patients with Otosclerosis.

机构信息

1 Department of Otorhinolaryngology and Head and Neck Surgery, Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra, Mexico City, Mexico.

2 Infectious Disease Division, Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra, Mexico City, Mexico.

出版信息

Otolaryngol Head Neck Surg. 2018 Jan;158(1):158-162. doi: 10.1177/0194599817733674. Epub 2017 Oct 3.

DOI:10.1177/0194599817733674

PMID:28971731

Abstract

Objective To determine molecularly the presence of measles virus genetic material in the stapes of patients with otosclerosis. Study Design A cross-sectional study. Setting A tertiary referral hospital. Subjects and Methods Genetic material was extracted from the stapes of patients with otosclerosis (n = 93) during the period from March 2011 to April 2012. The presence of viral measles sequences was evaluated by the real-time reverse transcriptase polymerase chain reaction (RT-PCR). The expression of the CD46 gene was determined. Results Ninety-three patients were included in the study. No sample was positive for any of 3 measles virus genes (H, N, and F). Measles virus RNA was not detected in any sample by real-time RT-PCR. CD46 levels were positive in 3.3% (n = 3) and negative in 96.7% (n = 90). Conclusion This study does not support the theory of measles virus as the cause of otosclerosis. It is necessary to do more research about other causal theories to clarify its etiology and prevention.

摘要

目的确定耳硬化症患者镫骨中麻疹病毒遗传物质的存在情况。

研究设计横断面研究。

设置三级转诊医院。

受试者和方法 2011 年 3 月至 2012 年 4 月期间，从 93 例耳硬化症患者的镫骨中提取遗传物质。通过实时逆转录聚合酶链反应 (RT-PCR) 评估病毒麻疹序列的存在情况。测定 CD46 基因的表达。

结果 93 例患者纳入研究。没有样本对任何 3 种麻疹病毒基因（H、N 和 F）呈阳性。实时 RT-PCR 未在任何样本中检测到麻疹病毒 RNA。CD46 水平阳性率为 3.3%（n = 3），阴性率为 96.7%（n = 90）。

结论本研究不支持麻疹病毒是导致耳硬化症的理论。有必要对其他因果理论进行更多研究，以阐明其病因和预防措施。