Sudjarwo Sri Agus, Sudjarwo Giftania Wardani
Faculty of Veterinary Medicine, Airlangga University, Surabaya 60115 Indonesia.
Faculty of Pharmacy, Hang Tuah University, Surabaya 60115 Indonesia.
Res Pharm Sci. 2017 Oct;12(5):381-390. doi: 10.4103/1735-5362.213983.
In recent years, the use of the antioxidant in reducing heavy metal toxicities has increased worldwide. Curcumin has been reported to have a strong antioxidant activity. In this study, we investigated the protective effects of curcumin on lead acetate-induced testicular damage in rats. The sample used 40 male rats divided into 5 groups: negative control (rats were given daily with corn oil); positive control (rats were given daily with lead acetate 50 mg/kg BW orally once in a day for 35 days); and the treatment group (rats were given the curcumin 100 mg, 200 mg, and 400 mg/kg BW orally once in a day for 40 days, and on the 5 day, were given lead acetate 50 mg/kg BW one h after the curcumin administration). After 40 days, levels of malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in testicular tissue, and sperm count, motility and viability in the epididymis were measured in rats. Testis samples were also collected for histopathological studies. Results showed that lead acetate administration significantly decreased the SOD, GPx, and increased MDA levels. Lead acetate also decreased the sperm count, motility, viability, and altered histopathological testis (testicular damage, necrosis of seminiferous tubules and loss of spermatid) compared to the negative control. However, administration of curcumin significantly improved the histopathological in testis, increased the sperm count, motility, viability, and also significantly increased the SOD, GPx, and decreased MDA in testis of lead acetate-treated rats. From the results of this study we concluded that the curcumin could be a potent natural product provide a promising protective effect against lead acetate induced testicular toxicity in rats.
近年来，抗氧化剂在全球范围内用于减轻重金属毒性的应用有所增加。姜黄素据报道具有很强的抗氧化活性。在本研究中，我们调查了姜黄素对醋酸铅诱导的大鼠睾丸损伤的保护作用。实验样本采用40只雄性大鼠，分为5组：阴性对照组（大鼠每日给予玉米油）；阳性对照组（大鼠每日口服50mg/kg体重的醋酸铅，持续35天）；治疗组（大鼠每日口服100mg、200mg和400mg/kg体重的姜黄素，持续40天，并在第5天，在给予姜黄素1小时后给予50mg/kg体重的醋酸铅）。40天后，测量大鼠睾丸组织中丙二醛（MDA）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）的水平，以及附睾中的精子数量、活力和存活率。还收集睾丸样本进行组织病理学研究。结果表明，给予醋酸铅显著降低了SOD、GPx水平，并增加了MDA水平。与阴性对照组相比，醋酸铅还降低了精子数量、活力、存活率，并改变了睾丸组织病理学（睾丸损伤、生精小管坏死和精子细胞丢失）。然而，给予姜黄素显著改善了睾丸的组织病理学，增加了精子数量、活力、存活率，并且还显著增加了醋酸铅处理大鼠睾丸中的SOD、GPx，并降低了MDA。从本研究结果我们得出结论，姜黄素可能是一种有效的天然产物，对醋酸铅诱导的大鼠睾丸毒性具有有前景的保护作用。
