Wu Haichong, Zhang Tao, Ma Xiaofei, Jiang Kangfeng, Zhao Gan, Qiu Changwei, Deng Ganzhen
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People's Republic of China.
Oncotarget. 2017 Jun 14;8(37):61487-61498. doi: 10.18632/oncotarget.18470. eCollection 2017 Sep 22.
IFN-τ is specifically secreted by the conceptus in ruminants during early pregnancy, and it plays a vital role in the immunological function of pregnancy. However, its mechanism involving microRNA (miRNA) is still not well understood. Deep sequencing was used to explore the specific miRNA library of IFN-τ on bovine endometrial epithelial cells (bEECs). The results showed that 574 known bovine miRNAs and 109 novel miRNAs were identified. We found 74 differentially expressed miRNAs, including 30 commonly expressed miRNAs in the experiment. Then, qPCR verification of six selected miRNAs showed that they corresponded with the sequencing data. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed significant enrichment of predicted target genes of differentially expressed miRNAs, including influenza A, herpes simplex infection, antigen processing and presentation, viral myocarditis, TNF signaling pathway, graft-versus-host disease, and allograft rejection. These results may provide important contributions to the immune response during early pregnancy in ruminants, but further studies are need to verify the proposed cellular/immunological effects and role of specific miRNA as biomarkers .
在反刍动物怀孕早期，干扰素τ（IFN-τ）由孕体特异性分泌，并且在妊娠免疫功能中发挥着至关重要的作用。然而，其涉及微小RNA（miRNA）的机制仍未被充分理解。利用深度测序技术探究IFN-τ作用于牛子宫内膜上皮细胞（bEECs）的特异性miRNA文库。结果显示，共鉴定出574个已知的牛miRNA和109个新的miRNA。我们发现了74个差异表达的miRNA，其中包括实验中30个共同表达的miRNA。随后，对六个选定miRNA进行的qPCR验证表明，它们与测序数据相符。基因本体论（GO）和京都基因与基因组百科全书（KEGG）通路分析显示，差异表达miRNA的预测靶基因显著富集，包括甲型流感、单纯疱疹感染、抗原加工与呈递、病毒性心肌炎、肿瘤坏死因子信号通路、移植物抗宿主病和同种异体移植排斥反应。这些结果可能对反刍动物怀孕早期的免疫反应有重要贡献，但需要进一步研究来验证所提出的特定miRNA作为生物标志物的细胞/免疫效应及作用。
