百日咳博德特氏菌自发释放的外膜囊泡的生理病理作用。
Physiopathological roles of spontaneously released outer membrane vesicles of Bordetella pertussis.
机构信息
GSK Vaccines, Via Fiorentina 1, 53100, Siena, Italy.
出版信息
Future Microbiol. 2017 Nov;12:1247-1259. doi: 10.2217/fmb-2017-0064. Epub 2017 Oct 5.
AIM
Bordetella pertussis has been shown to release outer membrane vesicles (OMV) both in vitro and in vivo but little is known about their biological role during the initial phases of B. pertussis infection of the airways.
RESULTS
We have demonstrated that OMV are released by B. pertussis in a human ciliated-airway cell model and purified vesicles can interact with host cells. Binding and uptake are strictly Bvg-regulated and OMV-associated pertussis toxin contributes to host-cell intoxication. Furthermore, we have shown that OMV act as iron-delivery systems complementing the B. pertussis growth defect in iron-limiting conditions.
CONCLUSION
We have proved that OMV play different roles in B. pertussis physiopathology and we opened new perspectives to be further investigated.
目的
已证明百日咳博德特氏菌在体外和体内都会释放外膜囊泡（OMV），但在百日咳博德特氏菌感染气道的初始阶段，它们的生物学作用知之甚少。
结果
我们已经证明，OMV 在人纤毛气道细胞模型中由百日咳博德特氏菌释放，纯化的囊泡可以与宿主细胞相互作用。结合和摄取严格受 Bvg 调节，并且 OMV 相关的百日咳毒素有助于宿主细胞中毒。此外，我们已经表明，OMV 作为铁传递系统发挥作用，补充铁限制条件下百日咳博德特氏菌的生长缺陷。
结论
我们已经证明 OMV 在百日咳博德特氏菌的病理生理学中发挥不同的作用，并为进一步研究开辟了新的视角。
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