南极地衣（盘菌纲，盘菌科）中真菌特异性简单序列重复标记
Fungus-specific SSR markers in the Antarctic lichens and (Parmeliaceae, Ascomycota).
作者信息
Lagostina Elisa, Dal Grande Francesco, Ott Sieglinde, Printzen Christian
机构信息
Department of Botany and Molecular Evolution, Senckenberg Research Institute and Natural History Museum Frankfurt, Senckenberganlage 25, D-60325 Frankfurt am Main, Germany.
Senckenberg Biodiversity and Climate Research Centre (BiK-F), Senckenberganlage 25, D-60325 Frankfurt am Main, Germany.
出版信息
Appl Plant Sci. 2017 Sep 28;5(9). doi: 10.3732/apps.1700054. eCollection 2017 Sep.
PREMISE OF THE STUDY
and (Parmeliaceae) are common lichens in the maritime Antarctic. These species share the same habitats on King George Island (South Shetland Islands, Antarctica) and are distinguishable based on reproductive strategies.
METHODS AND RESULTS
We developed 23 fungus-specific simple sequence repeat (SSR) markers that cross-amplify between the two species. We used a low-coverage genome-skimming approach on one sample of each species to identify SSR repeats in the two species. Primers were designed for 3-4-bp repeats, and only the loci common to both species were selected for further analyses. Seventy-seven samples of the two species were selected to assess fungal specificity, genetic variability, and linkage of the markers. In addition, we tested cross-amplification in other species.
CONCLUSIONS
The 23 newly designed SSR markers are suitable for population genetic and phylogeographic studies of species.
研究前提
地卷属（Parmeliaceae）的某些物种是南极海洋地区常见的地衣。这些物种在乔治王岛（南极南设得兰群岛）拥有相同的栖息地，并且可以根据繁殖策略加以区分。
方法与结果
我们开发了23个真菌特异性简单序列重复（SSR）标记，这些标记可在两个物种间进行交叉扩增。我们对每个物种的一个样本采用低覆盖度基因组鸟枪法测序方法，以鉴定这两个物种中的SSR重复序列。针对3 - 4碱基对重复序列设计引物，仅选择两个物种共有的位点进行进一步分析。选取这两个物种的77个样本，以评估真菌特异性、遗传变异性以及标记的连锁情况。此外，我们还在其他物种中测试了交叉扩增。
结论
新设计的23个SSR标记适用于地卷属物种的群体遗传学和系统地理学研究。
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