Department of Emergency, Central Hospital of Shengli Oil Field of Shandong, Dongying, Shandong 257000, P.R. China.
Department of Pediatrics, Central Hospital of Shengli Oil Field of Shandong, Dongying, Shandong 257000, P.R. China.
Mol Med Rep. 2017 Dec;16(6):8501-8506. doi: 10.3892/mmr.2017.7662. Epub 2017 Sep 29.
Ras homolog family member A (RhoA) has been reported to be involved in numerous biological processes; however, the effects of RhoA on acute lung injury (ALI) have yet to be reported. The present study aimed to explore how RhoA affects cell viability, reactive oxygen species (ROS) activity and cell apoptosis in a cell model of lipopolysaccharide (LPS)‑induced ALI. An MTT assay, flow cytometry, reverse transcription‑quantitative polymerase chain reaction and western blotting were used to determine the effects of RhoA on cell viability, apoptosis and ROS activity. The results demonstrated that RhoA inactivation was able to promote cell viability, and decrease apoptosis and ROS activity of LPS‑treated cells. The results of western blotting indicated that RhoA activated the downstream Wnt/β‑catenin signaling pathway and inhibited the expression of apoptotic factors. These findings suggested that RhoA may be involved in ALI progression and could be a novel therapeutic target for this disease.
Ras 同源家族成员 A（RhoA）已被报道参与许多生物过程；然而，RhoA 对急性肺损伤（ALI）的影响尚未见报道。本研究旨在探讨 RhoA 如何影响脂多糖（LPS）诱导的 ALI 细胞模型中的细胞活力、活性氧（ROS）活性和细胞凋亡。MTT 测定、流式细胞术、逆转录-定量聚合酶链反应和 Western blot 用于确定 RhoA 对细胞活力、凋亡和 ROS 活性的影响。结果表明，RhoA 失活能够促进 LPS 处理细胞的活力，并降低细胞凋亡和 ROS 活性。Western blot 结果表明，RhoA 激活了下游 Wnt/β-连环蛋白信号通路并抑制了凋亡因子的表达。这些发现表明，RhoA 可能参与 ALI 的进展，可能成为该疾病的新型治疗靶点。
