Sechler S R, Mullenix M K, Holland C M, Muntifering R B
J Anim Sci. 2017 Sep;95(9):3922-3931. doi: 10.2527/jas2017.1600.
A 2-yr study was conducted to determine effects of N fertilization level on fiber digestion kinetics and protein degradability characteristics of stockpiled Tifton 85 bermudagrass (T85). Six 0.76-ha pastures of stockpiled T85 were cut to a 10-cm stubble height on August 1 of each yr and fertilized with 56 (56N), 112 (112N), or 168 (168N) kg N/ha (2 pastures/treatment). Fiber digestion kinetics included the 72-hr potential extent of NDF digestion (PED), rate of NDF digestion, and lag time. In yr 1 and 2, PED decreased over the stockpile season. Rates of NDF digestion did not differ ( > 0.05) among N fertilization treatments in either yr. In yr 1, rate of NDF digestion was greatest ( < 0.05) in October/November, and decreased beginning in December. In yr 2, rate of NDF digestion decreased ( < 0.05) in January compared with November, but digestion rates were similar for November and January 21 sampling dates. Lag time was greater ( < 0.05) for the 112N than 56N and 168N treatments, and increased ( < 0.05) across sampling dates in yr 1. In yr 2, lag time increased ( < 0.05) from 9.0 to 17.7 h across the season. In yr 1 and 2, a negative correlation ( < 0.05) between forage lignin concentration and both PED ( = -0.91 and -0.87 in yr 1 and 2, respectively) and rate of NDF digestion ( = -0.60 and -0.25 in yr 1 and 2, respectively) was observed. There was a trend ( = 0.06) for lignin concentration to be positively correlated with lag time ( = 0.39) in yr 1, and a strong relationship was observed in yr 2 ( = 0.91; < 0.05). The RDP fraction as a % of CP was ≥ 90% throughout both years. Concentration of RDP (% of total DM) decreased across the stockpiling season through January in yr 1 and 2. Results suggest that kinetic parameters of NDF digestion in stockpiled T85 were influenced more by temporal changes over the stockpile season than by N fertilization level. Supplement formulations based on kinetic parameters of fiber digestion may require periodic adjustment to insure that energy-yielding components of NDF are sufficient to meet animal requirements throughout the stockpile season. The CP fraction in stockpiled T85 contains sufficient RDP to support fibrolytic activity and growth of ruminal microorganisms throughout the stockpile season. Toward the latter end of the season, supplementation with sources of digestible fiber and RDP could be expected to increase MP supply to the host animal.
开展了一项为期2年的研究,以确定氮肥施用量对堆贮的蒂夫顿85百慕大草(T85)纤维消化动力学及蛋白质降解特性的影响。每年8月1日,将6块0.76公顷的堆贮T85草地割至茬高10厘米,并分别施用56(56N)、112(112N)或168(168N)千克氮/公顷(每个处理2块草地)。纤维消化动力学指标包括72小时中性洗涤纤维消化潜在程度(PED)、中性洗涤纤维消化速率和延迟时间。在第1年和第2年,PED在堆贮季节均有所下降。两年中,不同氮肥处理间中性洗涤纤维消化速率无差异(P>0.05)。在第1年,中性洗涤纤维消化速率在10月/11月最高(P<0.05),12月开始下降。在第2年,1月的中性洗涤纤维消化速率较11月下降(P<0.05),但11月和1月21个采样日期的消化速率相似。112N处理的延迟时间长于56N和168N处理(P<0.05),且在第1年各采样日期延迟时间增加(P<0.05)。在第2年,整个季节延迟时间从9.0小时增加到17.7小时(P<0.05)。在第1年和第2年,牧草木质素浓度与PED(第1年和第2年分别为r = -0.91和-0.87)及中性洗涤纤维消化速率(第1年和第2年分别为r = -0.60和-0.25)均呈负相关(P<0.05)。在第1年,木质素浓度与延迟时间呈正相关趋势(r = 0.39,P = 0.06),第2年二者关系显著(r = 0.91,P<0.05)。两年中,可降解蛋白(RDP)占粗蛋白(CP)的比例均≥90%。在第1年和第2年,至1月整个堆贮季节,RDP浓度(占总干物质的百分比)均下降。结果表明,堆贮T85中性洗涤纤维消化动力学参数受堆贮季节时间变化的影响大于氮肥施用量的影响。基于纤维消化动力学参数的补充料配方可能需要定期调整,以确保中性洗涤纤维的能量产生成分在整个堆贮季节足以满足动物需求。堆贮T85中的CP部分含有足够的RDP,以支持整个堆贮季节瘤胃微生物的纤维分解活性和生长。在堆贮季节后期,补充可消化纤维和RDP有望增加宿主动物的代谢蛋白供应。