Kozulić B, Mosbach K, Pietrzak M
Institute of Biotechnology, ETH Hönggerberg, Zürich, Switzerland.
Anal Biochem. 1988 May 1;170(2):478-84. doi: 10.1016/0003-2697(88)90661-6.
Poly-N-acryloyl-tris(hydroxymethyl)aminomethane (NAT) gels were evaluated as a matrix for DNA electrophoresis. The resolution of DNA restriction fragments in three poly(NAT)-N,N'-methylenebisacrylamide (Bis) gels (4, 5, and 6%) was compared with the resolution in polyacrylamide (AA)-Bis gels of the same percentage. Poly(NAT) gels were found to give a substantially improved separation of DNA fragments larger than 200 bp. In contrast to poly(AA) gels, DNA fragments of up to 4 kbp were well resolved in the new matrix. By pulse-field electrophoresis the useful separation range of poly(NAT) gels was expanded to at least 23 kbp. For DNA fragments below 10 kbp, the resolution was better than that in a 0.7% agarose gel. Thus poly(NAT) gels are most suitable for the electrophoretic separation of DNA molecules whose size is out of the optimal fractionation range of poly(AA) or agarose gels.
聚-N-丙烯酰基-三（羟甲基）氨基甲烷（NAT）凝胶被评估作为DNA电泳的一种基质。将三种聚（NAT）-N，N'-亚甲基双丙烯酰胺（双）凝胶（4%、5%和6%）中DNA限制性片段的分辨率与相同百分比的聚丙烯酰胺（AA）-双凝胶中的分辨率进行了比较。发现聚（NAT）凝胶能显著改善大于200bp的DNA片段的分离效果。与聚（AA）凝胶不同，在新基质中高达4kbp的DNA片段能得到很好的分离。通过脉冲场电泳，聚（NAT）凝胶的有效分离范围扩大到至少23kbp。对于小于10kbp的DNA片段，分辨率优于0.7%琼脂糖凝胶。因此，聚（NAT）凝胶最适合用于电泳分离大小超出聚（AA）或琼脂糖凝胶最佳分级范围的DNA分子。
