通过静脉区域肢体灌注法给奶牛注射麻保沙星的药代动力学：两种不同止血带的评估

Pharmacokinetics of marbofloxacin administered via intravenous regional limb perfusion in dairy cows: evaluation of two different tourniquets.

作者信息

Celani Gianluca, Tulini Serena Maria Rita, Montesano Camilla, Zezza Daniela, Sergi Manuel, Varasano Vincenzo, Mortellaro Carlo Maria, Compagnone Dario, Amorena Michele, Petrizzi Lucio

机构信息

Faculty of Veterinary Medicine, Veterinary Teaching Hospital, University of Teramo, Teramo, Italy.

Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Teramo, Italy.

出版信息

Vet Rec Open. 2017 Sep 26;4(1):e000227. doi: 10.1136/vetreco-2017-000227. eCollection 2017.

DOI:10.1136/vetreco-2017-000227

PMID:29018533

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5623333/

Abstract

OBJECTIVES

This study evaluated synovial and systemic plasma pharmacokinetic variables of marbofloxacin after a single intravenous regional limb perfusion (IVRLP) performed using two different tourniquets in clinically healthy, standing, non-sedated dairy cows. The authors hypothesised that the type of tourniquet used for IVRLP would influence the synovial fluid concentration of marbofloxacin in the perfused distal limb.

DESIGN

The study had a randomised parallel-group design.

METHODS

Ten adult dairy cows were included. Unilateral hindlimb IVRLP through the dorsal common digital III vein was performed in two groups of five cows (group 1: wide rubber tourniquet; group 2: manual pneumatic tourniquet) using 0.67 mg/kg of marbofloxacin. The tourniquet was applied proximal to the tarsus and maintained for 30 minutes. Samples of jugular blood and synovial fluid from the tibiotarsal joints of the perfused limb were obtained before and at intervals after IVRLP. All samples were analysed for drug concentrations using liquid chromatography tandem mass spectrometry. Pharmacokinetic parameters were determined to establish the influence of tourniquet types. Differences were considered significant at P≤0.05.

RESULTS

No adverse effects from the procedure or marbofloxacin were observed in any animal. Significant differences in synovial concentrations and pharmacokinetic parameters were measured. The mean ± sd areas under the concentration versus time curve from time 0 to 24 hours were 178.98±58.08 µg hour/ml for group 2 and 21.11±9.93 µg hour/ml for group 1. The mean ± sd maximum marbofloxacin concentrations were 75.50±10.19 µg/ml for group 2 and 6.35±1.47 µg/ml for group 1.

CONCLUSIONS

Performing IVRLP using the dorsal common digital III vein and a manual pneumatic tourniquet set at 300 mmHg above the tarsus in standing cows resulted in significantly higher marbofloxacin concentrations in the tibiotarsal joint compared with those with the wide rubber tourniquet.

TRIAL REGISTRATION

Local ethical committee (number 41/2012/CEISA).

摘要

目的

本研究评估了在临床健康、站立、未镇静的奶牛中，使用两种不同止血带进行单次静脉区域肢体灌注（IVRLP）后，马波沙星在滑膜和全身血浆中的药代动力学变量。作者假设用于IVRLP的止血带类型会影响灌注远端肢体中马波沙星的滑液浓度。

设计

本研究采用随机平行组设计。

方法

纳入10头成年奶牛。两组各5头奶牛（第1组：宽橡胶止血带；第2组：手动气动止血带）通过背侧指总静脉III进行单侧后肢IVRLP，使用0.67mg/kg马波沙星。止血带应用于跗关节近端并维持30分钟。在IVRLP前及之后的不同时间间隔采集颈静脉血和灌注肢体胫跗关节的滑液样本。所有样本使用液相色谱串联质谱法分析药物浓度。确定药代动力学参数以评估止血带类型的影响。P≤0.05时差异被认为具有统计学意义。

结果

未在任何动物中观察到该操作或马波沙星产生的不良反应。测量到滑液浓度和药代动力学参数存在显著差异。第2组从时间0至24小时的浓度-时间曲线下平均±标准差面积为178.98±58.08μg·小时/ml，第1组为21.11±9.93μg·小时/ml。第2组马波沙星平均±标准差最大浓度为75.50±10.19μg/ml，第1组为6.35±1.47μg/ml。