Laboratory of Molecular Gerontology, National Institute on Aging, Intramural Research Program, National Institutes of Health, 251 Bayview Blvd., Ste. 100, Baltimore, MD, 21224, USA.
Molecular and Metabolic Oncology Program, USA Mitchell Cancer Institute, University of South Alabama, 1660 Springhill Avenue, Mobile, AL, 36604, USA.
Sci Rep. 2017 Oct 11;7(1):13007. doi: 10.1038/s41598-017-12963-7.
Base excision repair (BER) is the predominant pathway for coping with most forms of hydrolytic, oxidative or alkylative DNA damage. Measuring BER capacity in living cells is valuable for both basic science applications and epidemiological studies, since deficiencies in this pathway have been associated with cancer susceptibility and other adverse health outcomes. At present, there is an ongoing effort to develop methods to effectively quantify the rate of BER as a whole. We present a variation of a previously described "Oligonucleotide Retrieval Assay" designed to measure DNA excision repair that is capable of quantifying the rate of repair of thymine glycol in a variety of human cells with a high degree of sensitivity.
碱基切除修复（BER）是应对大多数水解、氧化或烷化 DNA 损伤的主要途径。在活细胞中测量 BER 能力对于基础科学应用和流行病学研究都很有价值，因为该途径的缺陷与癌症易感性和其他健康不良后果有关。目前，正在努力开发有效量化整体 BER 速率的方法。我们提出了一种先前描述的“寡核苷酸回收测定法”的变体，该方法旨在测量 DNA 切除修复，能够以高度敏感性定量各种人类细胞中胸腺嘧啶二醇的修复速率。
