20（S）-人参皂苷Rh2对小鼠γ-疱疹病毒的抗病毒活性。

Antiviral activity of 20()-ginsenoside Rh2 against murine gammaherpesvirus.

作者信息

Kang Soowon, Im Kyungtaek, Kim Geon, Min Hyeyoung

机构信息

College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea.

出版信息

J Ginseng Res. 2017 Oct;41(4):496-502. doi: 10.1016/j.jgr.2016.08.010. Epub 2016 Sep 1.

BACKGROUND

Ginsenosides are the major components of Meyer, an herbal medicine used for the treatment of various diseases. Different ginsenosides contribute to the biological properties of ginseng, such as antimicrobial, anticancer, and immunomodulatory properties. In this study, we investigated the antiviral effects of 15 ginsenosides and compound K on gammaherpesvirus.

METHODS

The antiviral activity of ginsenosides was examined using the plaque-forming assay and by analyzing the expression of the lytic gene.

RESULTS

20()-Ginsenoside Rh2 inhibited the replication and proliferation of murine gammaherpesvirus 68 (MHV-68), and its half-maximal inhibitory concentration (IC) against MHV-68 was estimated to be 2.77 μM. In addition, 20()-ginsenoside Rh2 inhibited 12--tetradecanoylphorbol-13-acetate (TPA)-induced lytic replication of human gammaherpesvirus in the Kaposi's sarcoma-associated herpesvirus (KSHV)-positive cell line BC3.

CONCLUSION

Our results indicate that 20()-ginsenoside Rh2 can inhibit the replication of mouse and human gammaherpesviruses, and thus, has the potential to treat gammaherpesvirus infection.

背景

人参皂苷是人参的主要成分，人参是一种用于治疗多种疾病的草药。不同的人参皂苷具有人参的生物学特性，如抗菌、抗癌和免疫调节特性。在本研究中，我们研究了15种人参皂苷和化合物K对γ疱疹病毒的抗病毒作用。

方法

使用噬斑形成试验并通过分析裂解基因的表达来检测人参皂苷的抗病毒活性。

结果

20()-人参皂苷Rh2抑制小鼠γ疱疹病毒68（MHV-68）的复制和增殖，其对MHV-68的半数最大抑制浓度（IC）估计为2.77μM。此外，20()-人参皂苷Rh2抑制12--十四酰佛波醇-13-乙酸酯（TPA）诱导的人γ疱疹病毒在卡波西肉瘤相关疱疹病毒（KSHV）阳性细胞系BC3中的裂解复制。